Research Article 
Detection of Polymorphisms in the Bovine Leptin (LEP) Gene: Association of a Single Nucleotide Polymorphism With Breeding Value of Milk Traits in Iranian Holstein Cattle
A. Javanmard, K. Khaledi, N. Asadzadeh and A.R. Solimanifarjam
Journal of Molecular Genetics, 2010, 2(1), 10-14.
Abstract
The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: Allele A was 390, 32 fragment and allele B was 303, 88 and 32 (only 303 fragment visible on the gel). Three patterns were observed frequencies were 0.900, 0.100 and 0.00 for AA, AB and BB, respectively (p≤0.21). The AB bulls were superior for milk fat production, in relation to homozygous AA. The Bovine leptin gene is strong candidates in the dairy cattle QTL search. This polymorphism could be further evaluated for marker assisted selection and the developed PCR methodology would expedite screening for large numbers of animal required for such studies.
ASCI-ID: 2638-11
Similar Articles
Barcoding of Five Sturgeon Species in Iran
Journal of Molecular Genetics, 2009, 1(2), 29-34.
Comparison of Rapid DNA Extraction Techniques for Conventional PCR-RFLP Analysis from Mammalian Whole Blood CellsJournal of Molecular Genetics, 2010, 2(3), 32-35.
Construction of a Vector Containing Coding Sequence of Lipid Transfer Proten-2 (LTP2) Gene from RiceJournal of Molecular Genetics, 2011, 3(1), 1-4.
Optimization the Production of the Sticky Ends of Human CCRL1 GeneJournal of Molecular Genetics, 2011, 3(1), 5-7.
Genetic Polymorphism Study of Promoter Region of Interleukin-2 Gene and its Association with Certain Milk Associated Traits in Indian Crossbred CattleJournal of Molecular Genetics, 2010, 2(1), 15-19.
Polymorphism of β-Lactoglobulin Gene in Iranian Sheep Breeds Using PCR-RFLPJournal of Molecular Genetics, 2010, 2(1), 6-9.
Characterization of Myostatin (MSTN) Gene in RabbitJournal of Molecular Genetics, 2015, 7(1), 1-4.
Cited By
Productive performance of the dairy cattle Girolando breed mediated by the fat-related genes DGAT1 and LEP and their polymorphisms
Research in Veterinary Science, 2011, (), . DOI: 10.1016/j.rvsc.2011.02.006
Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cowsInternational Journal of Dairy Technology, 2018, (), . DOI: 10.1111/1471-0307.12539
Insight in Leptin Gene Polymorphism and Impact on Milk Traits in Autochtonous Busha CattleActa Veterinaria, 2019, 69(2), 153. DOI: 10.2478/acve-2019-0012