Evaluation of Solid Lipid Nanoparticles Loaded with Praziquantel for Treatment of Schistosoma mansoni Infected Rats
Tohada M. AL-Noshokaty,
Dina M. Abo-Elmatty,
Noha M. Mesbah,
Ahmed S. Shehata
Research Journal of Parasitology,
2020, 15(2), 38-46.
Background and Objectives: Solid Lipid Nanoparticles (SLN) are a promising drug delivery system for oral administration of poorly water soluble drugs. Solid Lipid Nanoparticles (SLN) are at the forefront of the rapidly developing field of nanotechnology with several potential applications in drug delivery and research. The present work aimed to evaluate the efficacy of SLN loaded with praziquantel (PZQ) for treatment of schistosomiasis. Materials and Methods: The SLN was prepared and characterized to be a carrier for PZQ. Seventy male rats were divided equally into 7 groups (10 rats/group); normal control, infected control, infected and received SLN at 14 and 35 days, infected and received SLN loaded with PZQ which administered orally as two consecutive doses at days 14 and 35 post-infection, the last group infected and received PZQ. All rats were sacrificed after 8 weeks PI. Results: Liver section showed that treatment with SLN-PZQ the granuloma diameter was reduced. ALT and AST levels were decreased. Total Protein (TP) was increased and Gamma-glutamyl transferase was decreased. Level of IL-4 was decreased while, IL-10 level was high in treated group. Administration of PZQ at 35 days showed complete disappearance of ova. However, administration of SLN loaded with PZQ at 35 days post-infection decreased the worm burden and ova count in both liver and intestine compared to infected untreated group. Conclusion: Results of the present study conclude that the encapsulation of PZQ in SLN improved the safety profile of the drug. The PZQ in SLN could be a promising formulation to enhance the pharmacological activity of PZQ.
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