Research Article
Antioxidant, Analgesic and Anti-inflammatory Activities of the Herb Eclipta prostrata

M.S. Hossain, M.B. Alam, N.S. Chowdhury, M. Asadujjaman, R. Zahan, M.M. Islam, M.E.H. Mazumder, M.E. Haque and A. Islam

Journal of Pharmacology and Toxicology, 2011, 6(5), 468-480.

Abstract

The present study was designed to investigate the antioxidant, analgesic and anti-inflammatory potential of the methanolic extract along with its organic soluble fractions of the herb Eclipta prostrata. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,1diphenyl-2-picrylhydrazyl), NO (nitric oxide) and ONOO¯ (peroxynitrite) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.98±0.08, 45.98±0.07 and 14.45±0.18 μg mL-1 for DPPH, NO and ONOO- assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<0.001) activity. Further, the extract/fractions were studied for their analgesic (hot plate, tail immersion and acetic acid induced writhing test) and anti-inflammatory (carrageenan induced paw edema in rats) activities at a dose level of 200 and 400 mg kg-1 body weight. Among all the extract/fractions, EtOAc fraction showed a dose dependent and significant (p<0.005, p<0.05) analgesic activity in all the tested method. EtOAc fractions also reduced the paw edema considerably (86.80% inhibition after 3 h, p<0.005, p<0.05) in dose dependent manner compared to carrageenan induced rat. Altogether, these results suggest that the MeOH extract and its organic soluble fractions could be used as a potential antioxidant, analgesic and anti-inflammatory agent.

ASCI-ID: 55-350

Cited References Fulltext

Similar Articles


Anti Inflammatory, Antinociceptive and Central Nervous System Depressant Activities of Marine Bacterial Extracts

Journal of Pharmacology and Toxicology, 2009, 4(4), 152-159.

Evaluation of Antinociceptive and Antidiarrhoeal Properties of Pistia stratiotes (Araceae) Leaves

Journal of Pharmacology and Toxicology, 2011, 6(6), 596-601.

Nephrotoxicity Reduction by Ceftriaxone plus Vancomycin (Vancoplus) Reconstituted with VRP 1020 in Blood of Mus musculus Mice

Journal of Pharmacology and Toxicology, 2009, 4(3), 107-116.

Antioxidant, Anti-inflammatory and Anti-pyretic Activities of Trichosanthes dioica Roxb. Fruits

Journal of Pharmacology and Toxicology, 2011, 6(5), 440-453.

In vitro and in vivo Antioxidant and Toxicity Evaluation of Different Fractions of Oxalis corniculata Linn.

Journal of Pharmacology and Toxicology, 2011, 6(4), 337-348.

Evaluation of Antioxidant and Antiulcer Potentials of Prunus domestica Fruit Methanolic and Extract on Wistar Albino Rats

Journal of Pharmacology and Toxicology, 2012, 7(6), 305-311.

Anti-arthritic Effects of an Ethanolic Extract of Capparis erythrocarpos Isert Roots in Freund’s Adjuvant-induced Arthritis in Rats

Journal of Pharmacology and Toxicology, 2011, 6(3), 201-217.

Antioxidant, Anti-inflammatory and Anti-pyretic Activities of Trichosanthes dioica Roxb. Fruits

Journal of Pharmacology and Toxicology, 2011, 6(5), 440-453.

Cited By


CNS depressant activity of Castanopsis indica leaves

Oriental Pharmacy and Experimental Medicine, 2012, (), . DOI: 10.1007/s13596-012-0058-2

In vivo anti–nociceptive and anti–inflammatory activities of Lippia alba

Asian Pacific Journal of Tropical Disease, 2012, 2(), S667. DOI: 10.1016/S2222-1808(12)60241-2

Evaluation of Antioxidant and Wound Healing Potentials of Sphaeranthus amaranthoides Burm.f.

BioMed Research International, 2013, 2013(), 1. DOI: 10.1155/2013/607109

Pharmacognosy of Enicostemma littorale: A review

Asian Pacific Journal of Tropical Biomedicine, 2013, 3(1), 79. DOI: 10.1016/S2221-1691(13)60028-3

The efficacy of Carica papaya leaf extract on some bacterial and a fungal strain by well diffusion method

Asian Pacific Journal of Tropical Disease, 2012, 2(), S658. DOI: 10.1016/S2222-1808(12)60239-4

Rapid analysis of multi-pesticide residues in lotus seeds by a modified QuEChERS-based extraction and GC–ECD

Chemosphere, 2013, (), . DOI: 10.1016/j.chemosphere.2013.01.104

Acanthopanax koreanum Fruit Waste Inhibits Lipopolysaccharide-Induced Production of Nitric Oxide and Prostaglandin E2 in RAW 264.7 Macrophages

Journal of Biomedicine and Biotechnology, 2010, 2010(), 1. DOI: 10.1155/2010/715739

Attenuating Effect of Ginkgo biloba Leaves Extract on Liver Fibrosis Induced by Thioacetamide in Mice

Journal of Biomedicine and Biotechnology, 2012, 2012(), 1. DOI: 10.1155/2012/761450

Pretreatment Hepatoprotective Effect of the Marine Fungus Derived from Sponge on Hepatic Toxicity Induced by Heavy Metals in Rats

BioMed Research International, 2013, 2013(), 1. DOI: 10.1155/2013/510879

Effect of Indigenous Processing Methods on Phenolics and Antioxidant Potential of Underutilized Legumes Acacia auriculiformis and Parkia roxburghii

Journal of Food Quality, 2013, (), n/a. DOI: 10.1111/jfq.12024

Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes

Journal of Biomedicine and Biotechnology, 2012, 2012(), 1. DOI: 10.1155/2012/462049

Antioxidant and Hepatoprotective Effect of Aqueous Extract of Germinated and Fermented Mung Bean on Ethanol-Mediated Liver Damage

BioMed Research International, 2013, 2013(), 1. DOI: 10.1155/2013/693613

Acanthus ebracteatus Vahl. Ethanol Extract Enhancement of the Efficacy of the Collagen Scaffold in Wound Closure: A Study in a Full-Thickness-Wound Mouse Model

Journal of Biomedicine and Biotechnology, 2012, 2012(), 1. DOI: 10.1155/2012/754527

Antioxidant, analgesic and CNS depressant effects of Synedrella nodiflora

Pharmacognosy Journal, 2012, 4(31), 29. DOI: 10.5530/pj.2012.31.6

Beneficial health applications and medicinal values of Pedicularis plants: A review

Biomedicine & Pharmacotherapy, 2017, 95(), 1301. DOI: 10.1016/j.biopha.2017.09.041

Investigation of Nepetolide as a Novel Lead Compound: Antioxidant, antimicrobial, cytotoxic, anticancer, anti-inflammatory, analgesic activities and molecular docking

Saudi Pharmaceutical Journal, 2017, (), . DOI: 10.1016/j.jsps.2017.12.019