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Research Article
Antioxidant and in vitro Toxicity Assessment of Elegant Explosion Daylily Flower (Hemerocallis spp.) Extract in Hepatic Cell Line

J. Johnson, S. Ogutu and J. Boateng

Journal of Pharmacology and Toxicology, 2021, 16(1), 22-36.

Abstract

Background and Objective: Edible flowers such as the daylily (Hemerocallis spp.) have been shown to have beneficial effects against chronic diseases. This study determined antioxidant activity and polyphenol contents of two cultivars of daylily flowers Barbara Mitchell (BM) and Elegant Explosion (EE). The in vitro bioactivities of EE extract were further examined in HepG2 cell lines. Materials and Methods: BM and EE daylily flowers extracts were prepared with aqueous ethanol and used to determine Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total Monomeric Anthocyanins (TMA), Condensed Tannins (CT) and antioxidant assays. Selected phytochemicals were also determined via HPLC/DAD. In vitro toxicity, apoptosis and enzymatic activities of EE extracts were assessed in HepG2 cells. Results: Results indicated EE showed significantly (p<0.05) higher polyphenol contents and antioxidant activity compared to BM. In vitro cytotoxicity indicated that EE extracts possess moderately weak toxicity towards HepG2 cells. Significant (p<0.05) increases in antioxidant activities were noted. Conclusion: With limited studies on polyphenol content and bioactivity of EE daylily flower, these results provide preliminary evidence and contribute to the promotion of edible flowers as a potential dietary supplement and nutraceutical for use as functional foods.

ASCI-ID: 55-505

Cited References Fulltext

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