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Research Article
Evaluation of the Antidiabetic Effects of the Stem Bark Extract of Parinari curatellifolia (Planch. ex Benth.) in Drosophila melanogaster

S. Omale, J.C. Aguiyi, O.G. Adekunle, T.O. Johnson, S.O. Ochala, M.A. Etuh and M.C. Eze

Journal of Pharmacology and Toxicology, 2021, 16(1), 9-21.


Background and Objective: Type 2 diabetes is a chronic metabolic disorder of insulin resistance or insulin insensitivity characterized by sustained hyperglycemia with microvascular and macrovascular complications. Adult individuals with diabetes have two to three-fold increased risks of heart attacks, stroke, neuropathy, foot ulcers and eventual limb amputation. Drosophila melanogaster (fruit-fly) has been used as a model for investigating human diseases because of its genetic resemblance to humans. This study investigated the antidiabetic and antioxidant effect of ethanolic extract of the stembark of Parinari curatellifolia in D. melanogaster (Harwich strain). Materials and Methods: High-calorie diet of sucrose (2.5 g/10 g diet) was given to Drosophila for ten days to induce insulin resistance type 2 diabetes. The diabetic flies were then treated with 0.5, 1.0, 2.0 and 4.0 mg of P. curatellifolia per 10 g diet for seven days, 16 mg/10 g diet of metformin was used as standard drug. The glucose content of the fly-homogenate was quantified using glucose oxidase. Results: Furthermore, we determined the total thiol contents, Nitric Oxide (NO) levels, as well as the activities of catalase (CAT), Acetylcholinesterase (AChE) and glutathione-S-transferase (GST). We observed a significant decrease (p<0.05) in the glucose content of diabetic flies treated with extracts and standard drugs. Conclusion: There was a substantial decrease in the total thiol contents, NO levels, AChE activity and a significant increase in GST and CAT activities of the flies. These data are consistent with antidiabetic agents’ effects, indicating P. curatellifolia as a potential remedy for insulin-resistant type 2 diabetes.

ASCI-ID: 55-504

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