Research Article
Induction of Haploid Rice Plants Through in vitro Anther Culture

Sharmin Shahnewaz, M.A. Bari, N.A. Siddique, N. Khatun, M.H. Rahman and M.E. Haque

Pakistan Journal of Biological Sciences, 2003, 6(14), 1250-1252.

Abstract

Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinin). The best callusing from cultured anthers obtained in 2 medium containing 2,4-D 2 mg l-1, NAA 2.5 mg l-1 and kin 0.5 mg l-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg l-1, NAA 1 mg l-1, BAP 1.0 ml l-1 and incubated at 25±1OC in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.

ASCI-ID: 1-2808

Cited References Fulltext

Similar Articles


Mapping Quantitative Trait Loci Using the Marker Regression and the Interval Mapping Methods

Pakistan Journal of Biological Sciences, 2008, 11(4), 553-558.

Genetic Study of Mutations Induced by 3000 r Gamma Rays in Drosophila simulans

Pakistan Journal of Biological Sciences, 2001, 4(5), 559-561.

In silico Comparison of Simple Sequence Repeats in High Nucleotides-Rich Genomes of Microorganism

Pakistan Journal of Biological Sciences, 2008, 11(20), 2372-2381.

Analysis of Chromosome and Karyotype in Bali Cattle and Simmental-bali (Simbal) Crossbreed Cattle

Pakistan Journal of Biological Sciences, 2012, 15(15), 736-741.

Cytotoxic Effect of Aspartame (Diet Sweet) on the Histological and Genetic Structures of Female Albino Rats and Their Offspring

Pakistan Journal of Biological Sciences, 2012, 15(19), 904-918.

Chromosomal Studies and Quantitative Karyotypic Analysis of Rohu, Labeo rohita

Pakistan Journal of Biological Sciences, 2014, 17(4), 490-496.

Prevalence of BCR-ABL T315I Mutation in Different Chronic Myeloid Leukemia patients Categories

Pakistan Journal of Biological Sciences, 2022, 25(2), 175-181.

Effects of Different Ploidy Levels of Wheat (Hexaploids and Tetraploids) on Seed Set, Embryo Formation and Haploid Production in Wheat x Maize Crosses

Pakistan Journal of Biological Sciences, 2005, 8(12), 1758-1761.

Cited By


Indica rice anther culture: can the impasse be surpassed?

Plant Cell Tissue and Organ Culture, 2010, 100(1), 1. DOI: 10.1007/s11240-009-9616-9

Effects of Genotype on Induction of Callus and Plant Regeneration Potential in vitro Anther Culture of Rice (Oryza sativa L.)

Pakistan Journal of Biological Sciences, 2004, 7(2), 235. DOI: 10.3923/pjbs.2004.235.237

An improved anther culture procedure for obtaining new commercial Mediterranean temperate <i>japonica</i> rice (<i>Oryza sativa</i>) genotypes

Plant Biotechnology, 2018, (), . DOI: 10.5511/plantbiotechnology.18.0409a