Research Article
Immunological and Toxicological Studies of Staphylococcin Bac188 (A Bacteriocin/Bacteriocin-like Inhibitory Substance) on Experimental Animals

S. Saeed, S.A. Rasool, S. Ahmad and S.A Ali

Pakistan Journal of Biological Sciences, 2004, 7(11), 1888-1892.


Staphylococcin Bac188, a bacteriocin/Bacteriocin-like Inhibitory Substance (BLIS) from Staphylococcus aureus AB188 was studied for its immunological and toxicological effects on experimental animals. The antigenicity/immunogenicity of staphylococcin Bac188 was determined by Ouchterlony technique and Enzyme Linked Immunosorbent Assay (ELISA). Accordingly, staphylococcin Bac188 is antigenically poor (in rabbit immune system) as the antibody titer was not found to be significant in Ouchterlony test. However, some immunogenicity was witnessed in ELISA, suggesting that low level of antigen-antibody reaction has occurred. The acute and chronic toxic studies with Bac188 do not show any toxic effects in diseases virgin (domestic rabbits) when injected with different regimen of doses (20, 40, 60 and 80 μg mL-1). Hematological studies with staphylococcin Bac188 showed that except for marginal increase in lymphocyte count in the test animal groups, no other significant effect was observed as compared to the control (rabbits injected with plain sterile saline). Further, different doses of staphylococcin Bac188 showed no significant biochemical changes in the normal profile of rabbit blood biochemistry.

ASCI-ID: 1-3334

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