Pakistan Journal of Biological Sciences,
2005, 8(12), 1707-1711.
A significant effect of the plant development stage on fertility was observed at the time of colchicine treatment. Plants at
the 5-7 tillers stage showed improved plant fertility and reduced plant mortality than those treated at the 2-3 tillers
stage. The addition of colchicine to media resulted not only in higher plant fertility than did conventional methods, but
furthermore led to a huge increase in the number of doubled-haploid seeds.