Research Article 
Ex vitro Survival and Early Growth of Alpinia purpurata Plantlets Inoculated with Azotobacter and Azospirillum
I. Ovando-Medina, L. Adriano-Anaya, A. Chavez-Aguilar, A. Oliva-Llaven, T. Ayora-Talavera, L. Dendooven, F. Gutierrez-Miceli and M. Salvador-Figueroa
Pakistan Journal of Biological Sciences, 2007, 10(19), 3454-3457.
Abstract
The survival rate, shoot and root dry mass, shoot number, plant growth, stem height and diameter, number of leaves and root length were measured in micropropagated plantlets of Alpinia purpurata (Red ginger) inoculated with Azospirillum sp. 11B and Azotobacter sp. Pachaz 008 at 107, 108 and 109 cells cm3 using a complete randomized experimental design. Inoculation of A. purpurata plantlets with the Azospirillum sp. 11B or Azotobacter sp. PACHAZ 008 strains induced larger stem diameter, root dry mass, number of shoots and increased their survival rate from 77 to 100% compared to plantlets without inoculation, while other plant characteristics were not affected.
ASCI-ID: 1-4589
Similar Articles
Screening Plant Growth Promoting Rhizobacteria for Improving Seed Germination, Seedling Growth and Yield of Maize
Pakistan Journal of Biological Sciences, 2009, 12(1), 26-32.
Yield and Nitrogen Assimilation of Winter Wheat Inoculated with New Recombinant Inoculants of RhizobacteriaPakistan Journal of Biological Sciences, 2003, 6(4), 344-358.
Azotobacter and L-tryptophan Application for Improving Wheat YieldPakistan Journal of Biological Sciences, 1999, 2(3), 739-742.
Effect of Integrated Use of Mineral, Organic N and Azotobacter on the Yield, Yield Components and N-nutrition of Wheat (Triticum aestivum, L.)Pakistan Journal of Biological Sciences, 2003, 6(6), 539-543.
Enhancement of Growth and Nutrient Uptake of Rapeseed (Brassica napus L.) by Applying Mineral Nutrients and BiofertilizersPakistan Journal of Biological Sciences, 2009, 12(2), 127-133.
Yield and Nitrogen Assimilation of Winter Wheat Inoculated with New Recombinant Inoculants of RhizobacteriaPakistan Journal of Biological Sciences, 2003, 6(4), 344-358.
Substrate Dependent Microbially Derived Plant Hormones for Improving Growth of Maize SeedlingsPakistan Journal of Biological Sciences, 2000, 3(2), 289-291.
Effect of Azotobacter chroococcum Application on Quantity and Quality Forage of Rapeseed CultivarsPakistan Journal of Biological Sciences, 2007, 10(18), 3126-3130.
Testing Biological Agents and Methods to Control Fusarium Wilt of Alfalfa Plants (Medicage sativa L.)Pakistan Journal of Biological Sciences, 2004, 7(12), 2208-2211.
Effects of Azotobacter Application on the Growth and Yield of Transplant Aman Rice and Nutrient Status of Post-harvest SoilPakistan Journal of Biological Sciences, 2000, 3(7), 1144-1147.
Micropropagation of Aloe vera L. Grown in South IranPakistan Journal of Biological Sciences, 2007, 10(7), 1134-1137.
Shoot Micropropagation and Microtuberization in Potato (Solanum tuberosum L.) By the Semi-Continuous BioreactorPakistan Journal of Biological Sciences, 2007, 10(6), 861-867.
Micropropagation of Annona squamosa Linn. Using Explants (Shoot Tip and Node) of Field Grown Mature PlantsPakistan Journal of Biological Sciences, 2002, 5(4), 394-397.
In vitro Propagation of Rosa indicaPakistan Journal of Biological Sciences, 2003, 6(9), 826-830.
Micropropagation of Woody Legume (Albizia lebbeck) Through Tissue CulturePakistan Journal of Biological Sciences, 2004, 7(7), 1099-1103.
Plantlet Regeneration Potential from Seedling Explants of Vitegnus (Vitex agnus castus)Pakistan Journal of Biological Sciences, 2007, 10(22), 4113-4117.
In vitro Micropropagation of Desmodium gangeticum (L.) DC (Fam-Fabaceae): A Medicinal Legume through Axillary Bud MultiplicationPakistan Journal of Biological Sciences, 2012, 15(10), 477-483.
In vitro Propagation and Cryopreservation of Aerides odorata Lour. (Orchidaceae)Pakistan Journal of Biological Sciences, 2014, 17(5), 608-618.
Micropropagation of Dalbergia sissoo Roxb. Through Tissue Culture TechniquePakistan Journal of Biological Sciences, 2014, 17(4), 597-600.
Effect of AgNO3 and BAP on Root as a Novel Explant in Date Palm (Phoenix dactylifera cv. Medjool) Somatic EmbryogenesisPakistan Journal of Biological Sciences, 2017, 20(1), 20-27.
Effect of Different Hormonal Treatment on Stevia (rebaudiana Bertoni) Micro-propagationPakistan Journal of Biological Sciences, 2017, 20(9), 457-464.
A Protocol for High Frequency Regeneration Through Nodal Explant Cultures and ex vitro Rooting of Plumbago rosea L.Pakistan Journal of Biological Sciences, 2007, 10(2), 349-355.
DNA Fingerprinting of in vitro Micropropagated Pomegranate GenotypesPakistan Journal of Biological Sciences, 2020, 23(5), 619-627.
Micropropagation and Secondary Metabolites Content of White-Purple Varieties of Orthosiphon aristatus Blume Miq.Pakistan Journal of Biological Sciences, 2021, 24(8), 858-867.
In vitro Culture Optimization of Pomelo Seeds (Citrus maxima (Burm.) Merr.): A South Sulawesi OrangePakistan Journal of Biological Sciences, 2023, 26(11), 576-585.
In vitro Propagation of Ginger (Zingiber officinale Rosc.) through Direct Organogenesis: A ReviewPakistan Journal of Biological Sciences, 2013, 16(24), 1826-1835.
In vitro Micropropagation of Anthemis xylopoda O. Schwarz, a Critically Endangered Species from TurkeyPakistan Journal of Biological Sciences, 2005, 8(5), 691-695.
Micropropagation of Annona squamosa L. Using Nodal ExplantsPakistan Journal of Biological Sciences, 2002, 5(1), 43-46.
An Efficient and Economical Method of Mass Multiplication of Virus and Disease Free Banana Using Plant Tissue Culture TechniquesPakistan Journal of Biological Sciences, 2001, 4(5), 562-563.
Plantlet Regeneration from Callus Cultures of Kappaphycus alvarezii for Cultivation in Coastal Waters at Myeik Archipelago, MyanmarPakistan Journal of Biological Sciences, 2024, 27(9), 479-486.
Comparative Growth of Red Chrysanthemum Plantlets in Micropropagation and Microponic SystemsPakistan Journal of Biological Sciences, 2026, 29(1), 33-47.
Cited By
Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
Plant Cell, Tissue and Organ Culture (PCTOC), 2017, 130(1), 37. DOI: 10.1007/s11240-017-1202-y
Commercial use of endophytes in micropropagationActa Horticulturae, 2017, (1155), 483. DOI: 10.17660/ActaHortic.2017.1155.71
Interaction between Piriformospora indica and Azotobacter chroococcum governs better plant physiological and biochemical parameters in Artemisia annua L. plants grown under in vitro conditionsSymbiosis, 2018, 75(2), 103. DOI: 10.1007/s13199-017-0519-y
Handbook of Research on Principles and Practices for Orchards ManagementPractice, Progress, and Proficiency in Sustainability, 2022, (), 203. DOI: 10.4018/978-1-6684-2423-0.ch012
Guarana propagation strategies: a reviewBrazilian Journal of Biology, 2023, 83(), e275940. DOI: 10.1590/1519-6984.275940
Effects of Ornamental Plant Density and Mineral/Plastic Media on the Removal of Domestic Wastewater Pollutants by Home Wetlands TechnologyMolecules, 2020, 25(22), 5273. DOI: 10.3390/molecules25225273
Effect of Azospirillum brasilense on the in vitro germination of Eustoma grandiflorum (Raf.) Schinn.(Gentianaceae)Scientia Horticulturae, 2022, 299(), 111041. DOI: 10.1016/j.scienta.2022.111041