Research Article 
Study of Two Main Approaches-Electrophoresis and Chromatography as Varietal Identification Methods in Rice
M. Shokrzadeh, P. Valipour and A.G. Ebadi
Pakistan Journal of Biological Sciences, 2007, 10(3), 439-445.
Abstract
Rice is a major worldwide crop that cultivated in the most areas of the north of Iran (Mazandaran and Gillan Province). An increase in the assortment of rice varieties is making it progressively more difficult to distinguish between the many cultivars by traditional visual identification methods. The more advanced identification techniques of electrophoresis and chromatography offer an effective solution to this emerging identification dilemma. This paper reviews the application of these two evaluation techniques. An Electrophoresis analysis includes gel electrophoresis and capillary electrophoresis and compares them with a popular chromatography technique, namely reversed-phase, size exclusion anion-exchange high performance liquid chromatography (HPLC). This paper will also include an interpretation of the results.
ASCI-ID: 1-335
Similar Articles
Isolation Identification of Cysteine Protease Cathepsin S From Breast Cancerous Tissue
Pakistan Journal of Biological Sciences, 2000, 3(3), 508-510.
Sequential Alignment and 3rd Structure of Serine Protease Granzyme HPakistan Journal of Biological Sciences, 2001, 4(1), 110-111.
Physio-chemical Characterization and Anti-microbial Activity of Serine Protease Inhibitors Purified from the Sophora japonica SeedsPakistan Journal of Biological Sciences, 2018, 21(9), 432-440.
Molecular Aspects of Drought Tolerance in Bread Wheat (T. aestivum)Pakistan Journal of Biological Sciences, 2008, 11(1), 118-122.
An Electrophoretic Analysis of the Seed Proteins of some Vicia L. Species from Northeast Anatolia (Turkey)Pakistan Journal of Biological Sciences, 1999, 2(4), 1139-1142.
Isolation Identification of Cysteine Protease Cathepsin S From Breast Cancerous TissuePakistan Journal of Biological Sciences, 2000, 3(3), 508-510.
Sequential Alignment and 3rd Structure of Serine Protease Granzyme HPakistan Journal of Biological Sciences, 2001, 4(1), 110-111.
The Significance of Cuticular Features, Petiole Anatomy and SDS-PAGE in the Taxonomy of the LauraceaePakistan Journal of Biological Sciences, 2001, 4(9), 1094-1100.
Studies on the Rosaceae II- SDS-PAGE Seed Protein Electrophoresis and its Significance in the Taxonomy of the FamilyPakistan Journal of Biological Sciences, 2003, 6(21), 1820-1829.
Genetic Polymorphism of Blood Proteins in Iranian Kurd and Turkoman Horse PopulationsPakistan Journal of Biological Sciences, 2006, 9(1), 155-159.
Isozyme analysis of seedling samples in some species of Hyoscyamus from IranPakistan Journal of Biological Sciences, 2006, 9(9), 1685-1692.
Isolation, Identification and 3D Structure of Extracellular Lipase from Mucor hiemalis by Gel ElectrophoresisPakistan Journal of Biological Sciences, 2000, 3(3), 511-512.
Genetic Relatedness of Vibrio cholerae O1 and non-O1 by ERIC Polymerase Chain Reaction and Pulsed-Field Gel ElectrophoresisPakistan Journal of Biological Sciences, 2000, 3(4), 621-622.
Inter and Intra-specific Variation in SDS-PAGE Electrophoregrams of Total Seed Protein in Wheat, Barley and Their Wild SpeciesPakistan Journal of Biological Sciences, 2000, 3(12), 2223-2225.
Accumulation and Distribution of Ni63 by Maize Seedlings Grown in Labeled Soils Amended with SludgePakistan Journal of Biological Sciences, 2002, 5(2), 135-142.
Estimation of Combining Ability for Yield and Yield Components in Rice(Oryza sativa L.) Cultivars Using Diallel CrossPakistan Journal of Biological Sciences, 2008, 11(9), 1278-1281.
An Assessment of Wx Microsatellite Allele, Alkali Degradation and Differentiation of Chloroplast DNA in Traditional Black Rice (Oryza sativa L.) From Thailand and Lao PDRPakistan Journal of Biological Sciences, 2007, 10(2), 261-266.
Effect of Zinc Application on Rice Yield under Wheat Rice SystemPakistan Journal of Biological Sciences, 2007, 10(2), 235-239.
Agronomic and Quality Characteristics of High-Yielding Rice LinesPakistan Journal of Biological Sciences, 2007, 10(1), 142-144.
Response of Rice Cultivars to Rates of Nitrogen and Potassium Application in Field and Pot ConditionsPakistan Journal of Biological Sciences, 2007, 10(9), 1430-1437.
Effects of N and K Applications on Agronomic Characteristics of Two Iranian and Landrace Rice (Oryza sativa L.) CultivarsPakistan Journal of Biological Sciences, 2007, 10(6), 880-886.
Identification of Variant Transcripts of Waxy Gene in Non-glutinous Rice (O. sativa L.) With Different Amylose ContentPakistan Journal of Biological Sciences, 2007, 10(15), 2500-2504.
Phosphorus Fertilization for Rice and Control of Environmental Pollution ProblemsPakistan Journal of Biological Sciences, 2007, 10(13), 2098-2105.
Effect of an Auxin Precursor L-tryptophan on Growth and Yield of Rice (Oryza stiva L.)Pakistan Journal of Biological Sciences, 1998, 1(4), 354-356.
Effect of Seedling Age and Density on Growth and Yield of Rice in Saline SoilPakistan Journal of Biological Sciences, 1999, 2(3), 860-862.
Niab-Irri-9: A New Salt Tolerant and High Yielding Rice VarietyPakistan Journal of Biological Sciences, 1999, 2(3), 869-870.
Improvement of Nitrogen Fertilizer Efficiency with Nitrification Inhibitors in Lowland RicePakistan Journal of Biological Sciences, 1999, 2(4), 1184-1187.
Entomocidal Activity of Bacillus Thuringiensis Transgenic Rice Plants Against Rice Leaffolder, Cnaphalocrocis Medinalis (Lepidoptera: Pyralidae).Pakistan Journal of Biological Sciences, 1999, 2(4), 1472-1477.
Performance of Six Rice Cultivars Under Direct Wet Seeded Conditions of Dera Ismail KhanPakistan Journal of Biological Sciences, 1999, 2(4), 1504-1506.
Growth and Yield Response of Basmati-385 (Oryza sativa L.) to ZnSO4 ApplicationPakistan Journal of Biological Sciences, 1999, 2(4), 1632-1633.
Effect of Different Clipping Heights on the Green Fodder, Hay and Seed Production of Four Cultivars of Transplant Aman RicePakistan Journal of Biological Sciences, 2001, 4(2), 156-158.
Effect of Tree Prunings on Soil Fertility and Crop Yield in Alley Cropping SystemPakistan Journal of Biological Sciences, 2001, 4(6), 647-650.
Direct Seeded Rice (Dsr) Genotypes for Drought Prone Upland AreaPakistan Journal of Biological Sciences, 2001, 4(6), 651-653.
Effect of Agronomic Practices on the Production of Green Fodder, Hay and Seed Yield of RicePakistan Journal of Biological Sciences, 2001, 4(8), 931-933.
Effect of Pre-flowering Leaf Cutting on Forage and Seed Yield of Transplant Aman RicePakistan Journal of Biological Sciences, 2001, 4(8), 934-936.
Effect of Nitrogen Rate and Time of Leaf Cutting on Green Fodder as Well as Seed Yield of RicePakistan Journal of Biological Sciences, 2001, 4(8), 937-939.
Physico-chemical Changes in Different Varieties of Rice as Influenced by the Application of Blue-green AlgaePakistan Journal of Biological Sciences, 2001, 4(11), 1306-1311.
Seedling Establishment of an Anoxia-tolerant Rice Genotype as Affected by Soil Source and Days after SubmergencePakistan Journal of Biological Sciences, 2001, 4(12), 1495-1497.
Seasonal Effect of Seedling Age on the Yield of RicePakistan Journal of Biological Sciences, 2002, 5(1), 40-42.
Organic Acids Toxicity on Seedling Attributes of Anoxia Tolerant Rice Genotypes Grown in HypoxiaPakistan Journal of Biological Sciences, 2002, 5(1), 36-39.
Relative Contribution of the Coleoptile and the First Leaf Length to Seedling Establishment of Rice (Oryza sativa L.) as Affected by Anaerobic Seeding in Two Different SoilsPakistan Journal of Biological Sciences, 2002, 5(4), 413-415.
Initiation and Cryopreservation of Cell Suspension of Rice Basmati VarietiesPakistan Journal of Biological Sciences, 2002, 5(6), 679-682.
Studies on Parental Variability and Heterosis in RicePakistan Journal of Biological Sciences, 2002, 5(10), 1006-1009.
Effect of NaCl and PEG Induced Osmotic Potentials on Germination and Early Seedling Growth of Rice Cultivars Differing in Salt TolerancePakistan Journal of Biological Sciences, 2002, 5(11), 1207-1210.
Water Uptake and Germination Pattern of Rice Seeds under Iso-osmotic Solutions of NaCl and Peg, Different Concentrations of CaCl2 and Combinations of NaCl and CaCl2Pakistan Journal of Biological Sciences, 2003, 6(12), 1059-1066.
Effect of a Range of Constant Temperatures on Germination of Fifteen Bangladeshi Rice (Oryza sativa L.) CultivarsPakistan Journal of Biological Sciences, 2003, 6(12), 1070-1076.
Inter and Intra-Specific Variation in SDS-PAGE of Total Seed Protein in Rice (Oryza sativa L.) GermplasmPakistan Journal of Biological Sciences, 2004, 7(2), 139-143.
Effect of Salinity on Some Yield Attributes of RicePakistan Journal of Biological Sciences, 2004, 7(5), 760-762.
Effect of Alternating Saline and Non-saline Conditions on Emergence and Seedling Growth of RicePakistan Journal of Biological Sciences, 2004, 7(6), 883-890.
Prevalence of Rice Root Nematode in the Farmer Rice Fields as Influenced by Edaphic FactorsPakistan Journal of Biological Sciences, 2004, 7(11), 1837-1839.
Population Dynamics of Hirschmanniella oryzae in the Rice Root of Farmer Fields as Affected by Edaphic FactorsPakistan Journal of Biological Sciences, 2004, 7(11), 2002-2004.
Yield and Biomass in Rice Interactions of Nitrogen, Phosphorous and Water ApplicationPakistan Journal of Biological Sciences, 2004, 7(12), 2115-2120.
Effect of Water Saving Irrigation on Yield and Concentration of Ca and Mg in Malaysian Rice CultivationPakistan Journal of Biological Sciences, 2005, 8(1), 65-67.
Accumulation of Cu in Root Apoplasm and Re-translocation to Young Leaves in Rice, Maize and Sunflower with Different Toxicity TolerancePakistan Journal of Biological Sciences, 2005, 8(11), 1599-1609.
In vitro Plant Regeneration Through Anther Culture of Some Iranian Local Rice (Oryza sativa L.) CultivarsPakistan Journal of Biological Sciences, 2007, 10(12), 2056-2060.
Investigation the Growth, Yield and Yield Components of Rice Varieties in Rotation with Garlic, Faba Bean, Lettuce, Pea and Fallow in North of IranPakistan Journal of Biological Sciences, 2007, 10(16), 2688-2693.
Effect of Planting Density on Agronomical Characteristics of Rice (Oryza sativa L.) Varieties in North of IranPakistan Journal of Biological Sciences, 2007, 10(18), 3205-3209.
Effect of Variable Rates of Gypsum Application on Wheat Yield Under Rice-Wheat SystemPakistan Journal of Biological Sciences, 2007, 10(21), 3865-3869.
Identification of Salt Tolerant Rice Cultivars Via Phenotypic and Marker-Assisted ProceduresPakistan Journal of Biological Sciences, 2007, 10(24), 4449-4454.
The Perspective Effects of Various Seed Coating Substances on Rice Seed Variety Khao Dawk Mali 105 Storability II: The Case Study of Chemical and Biochemical PropertiesPakistan Journal of Biological Sciences, 2009, 12(7), 574-581.
Rice Optimal Water Use in Different Air Temperatures at Flowering, Nitrogen Rates and Plant PopulationsPakistan Journal of Biological Sciences, 2007, 10(23), 4197-4203.
Genetic Architecture, Inter-relationship and Selection Criteria for Yield Improvement in Rice (Oryza sativa L.)Pakistan Journal of Biological Sciences, 2011, 14(9), 540-545.
Performance of Different Rice Cultivars at Farmers` Field under the Agro-climatic Conditions of Dera Ismail KhanPakistan Journal of Biological Sciences, 2003, 6(4), 314-316.
Zinc and Salinity Interaction on Agronomical Traits, Chlorophyll and Proline Content in Lowland Rice (Oryza sativa L.) GenotypesPakistan Journal of Biological Sciences, 2006, 9(7), 1315-1319.
Genetic Evaluation of Recombinant Inbred Lines of Rice (Oryza sativa L.) for Grain Zinc Concentrations, Yield Related Traits and Identification of Associated SSR MarkersPakistan Journal of Biological Sciences, 2013, 16(23), 1714-1721.
Pest and Disease Tolerance in Rice cv Pusa Basmati as Related to Different Locally Available Organic Manures Grown in New Alluvail Region of West Bengal, IndiaPakistan Journal of Biological Sciences, 2013, 16(12), 593-597.
Rice Tungro Disease in Sarawak: Past and Present StatusPakistan Journal of Biological Sciences, 2015, 18(6), 285-289.
Physio-chemical, Properties, Adoption and Productivity of Some Fine (Basmati) Rice Varieties in Dera Ismail KhanPakistan Journal of Biological Sciences, 1998, 1(4), 264-266.
Evaluation Of Compost Fertilizer "Zarkhez" In Conjunction With Chemical Fertilizers For Rice And Wheat ProductionPakistan Journal of Biological Sciences, 1998, 1(4), 368-371.
Role of Plant Growth Regulators (Auxin and Cytokinin) in Callus Induction in Rice (Oryza sativa L.) C.V. DM-25Pakistan Journal of Biological Sciences, 2000, 3(1), 157-159.
Germination and Seedling Growth of Rice (Oryza sativa L.) Under Saline ConditionsPakistan Journal of Biological Sciences, 2000, 3(2), 350-351.
Genetic Variability among Different Rice Cultivars for Phosphorus Uptake and UtilizationPakistan Journal of Biological Sciences, 2000, 3(5), 881-882.
Genetic Variability among Different Rice Cultivars for Zinc Uptake and UtilizationPakistan Journal of Biological Sciences, 2000, 3(7), 1174-1176.
Genetic Variation for Phosphorus Use in Rice at Two Levels of Soil Applied PhosphorusPakistan Journal of Biological Sciences, 2000, 3(8), 1274-1276.
Natural Variation among Rice Yellow Stem Borer, Scirpophaga incertulas (Walker) and Rice Leaffolder, Cnaphalocrocis medinalis (Guenee) Populations to Bacillus thuringiensis δ-endotoxinsPakistan Journal of Biological Sciences, 2000, 3(8), 1285-1289.
Screening for Putative Transgenic Rice and Cotton Plants: A Simple and Easy MethodPakistan Journal of Biological Sciences, 2000, 3(12), 2226-2228.
Effect of Sodium Chloride Salinity on Seed Germination and Early Seedling Growth of Rice (Oryza sativa L.)Pakistan Journal of Biological Sciences, 2001, 4(3), 351-355.
Effect of Tillage Practices and Nitrogen Rates on the Nitrogen Content and Nitrogen Uptake in Rice Var. BinasailPakistan Journal of Biological Sciences, 2003, 6(14), 1202-1204.
Nodulation in Cereals as a Means to Decreasing Their Dependence on Nitrogenous Fertilizers - An Achievable Target or a DogmaPakistan Journal of Biological Sciences, 2002, 5(1), 122-127.
Effect of Seed Cleaning, Washing and Seed Treatment on Seedling Disease Incidence and Yield of RicePakistan Journal of Biological Sciences, 2002, 5(7), 767-769.
Effect of Plant Extracts Against Bipolaris oryzae of Rice under In vitro ConditionsPakistan Journal of Biological Sciences, 2002, 5(4), 442-445.
Genetic Diversity in Irrigated RicePakistan Journal of Biological Sciences, 2004, 7(2), 226-229.
Identification and Pathogenicity of Fungal Dieback Disease on Sengon (Paraserianthes falcataria (L.) Nielsen) Seedling and Rice (Oryza sativa)Pakistan Journal of Biological Sciences, 2018, 21(1), 16-23.
Control of Rice Powder Pest Sitophilus oryzae L. with Entomopathogenic Fungus Metarhizium anisopliae (Metch.)Pakistan Journal of Biological Sciences, 2023, 26(2), 56-62.