Research Article 
Antioxidative Responses in Calli of Two Populations of Acanthophyllum laxiusculum With and Without B-chromosomes under Salt Stress
A.A. Meratan, S.M. Ghaffari, V. Niknam and M. Salami
Pakistan Journal of Biological Sciences, 2013, 16(1), 1-11.
Abstract
Salinity is one of the most significant stresses that affect plant growth and agricultural production. Considerable efforts have therefore been made to investigate how plants respond to salt stress. To investigate the responses of Acanthophyllum to salinity stress, changes on lipid peroxidation, H2O2 content and the activity of antioxidant enzymes in calli of two Acanthophyllum laxiusculum variants, without (variant A) and with (variant B) B-chromosomes, were analyzed. Under salinity stress, lipid peroxidation and H2O2 content decreased in variant A compared to variant B. Moreover, variant A produced a higher amount of proline under salt stress than variant B. In calli of variant A, salinity stress preferentially enhanced the activities of the superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC 1.6.4.2), whereas it decreased their activity in variant B. Guaiacol peroxidase (GPX, EC 1.11.1.7) activity decreased with increasing NaCl concentrations in variant A but its activity increased in variant B. After native polyacrylamide gel electrophoresis (PAGE) analysis, calli of both A and B variants had only Mn-SOD and Fe-SOD isoforms. Expression of the isoforms mentioned above, showed marked increase in variant A; while in variant B salinity caused a significant reduction as compared to control. Moreover, changes in the CAT and GPX isoforms pattern of treated plants were in accordance with the activity changes in time. These results suggest that variant A was able to induce better antioxidative responses against salt stress than variant B.
ASCI-ID: 1-5874
Similar Articles
Antioxidant Responses of Two Barley Varieties to Saline Stress
Pakistan Journal of Biological Sciences, 2008, 11(6), 905-909.
Combined Supplementation of Soy and Garlic modulate Biochemical Parameters of 7,12-dimethylbenz[α]anthracene Induced Mammary Cancer in Female Albino RatsPakistan Journal of Biological Sciences, 2007, 10(14), 2308-2313.
The Effect of Different Concentrations of Salicylic Acid on Protective Enzyme Activities of Pepper (Capsicum annuum L.) PlantsPakistan Journal of Biological Sciences, 2007, 10(18), 3162-3165.
Ameliorating Role of Rutin on Oxidative Stress Induced by Iron Overload in Hepatic Tissue of RatsPakistan Journal of Biological Sciences, 2014, 17(8), 964-977.
Allelopathic Potentials of Artrmisia monosperma and Thymus vulgaris on Growth and Physio-Biochemical Characteristics of Pea SeedlingsPakistan Journal of Biological Sciences, 2018, 21(4), 187-198.
Seed Dressing Affect Protein, Antioxidant Enzymes, Ultrastructure, Soil Properties and Microflora of Maize and Wheat SeedlingsPakistan Journal of Biological Sciences, 2020, 23(6), 782-794.
Impact Fed Larvae on Residues (Tramadol, Sildenafil Citrate and Diazepam) Chrysomya albiceps on EnzymesPakistan Journal of Biological Sciences, 2022, 25(8), 705-714.
Copper-Induce Change in Antioxidative System in Maize (Zea mays L.)Pakistan Journal of Biological Sciences, 2007, 10(20), 3662-3667.
Prevention of CCl4-Induced Liver Damage by Ginger, Garlic and Vitamin EPakistan Journal of Biological Sciences, 2007, 10(4), 617-621.
Differential Responses of Two Tomato Cultivars (Lycopersicon esculentum L.) to NaCl StressPakistan Journal of Biological Sciences, 2001, 4(12), 1456-1459.
Comparative Effects of Copper and Cadmium on Growth and Lipid Content in Maize Seedlings (Zea mays L.)Pakistan Journal of Biological Sciences, 2005, 8(4), 649-655.
Hepatoprotective Activity of Hibiscus cannabinus (Linn.) Against Carbon Tetrachloride and Paracetamol Induced Liver Damage in RatsPakistan Journal of Biological Sciences, 2005, 8(10), 1397-1401.
β-carotene Protects the Physiological Antioxidants Against Aflatoxin-B1 Induced Carcinogenesis in Albino RatsPakistan Journal of Biological Sciences, 2006, 9(6), 1104-1111.
The Effect of Water Stress on the Antioxidant Content, Protective Enzyme Activities, Proline Content and Lipid Peroxidation in Wheat SeedlingPakistan Journal of Biological Sciences, 2008, 11(15), 1916-1922.
Effects of Amodiaquine Hydrochloride and Artemisinin on Indomethacin-Induced Lipid Peroxidation in RatsPakistan Journal of Biological Sciences, 2008, 11(17), 2154-2158.
Free Radical Scavenging and Lipid Peroxidation Activity of the Shahani Black GrapePakistan Journal of Biological Sciences, 2008, 11(21), 2513-2516. DOI:
Biochemical Changes of Hemoglobin and Osmotic Fragility of Red Blood Cells in High Fat Diet RabbitsPakistan Journal of Biological Sciences, 2010, 13(2), 73-77.
Lipid Peroxidation Alterations in Type 2 Diabetic PatientsPakistan Journal of Biological Sciences, 2010, 13(15), 723-730.
Effects of Salicylic Acid on Growth, Biochemical Constituents in Pepper (Capsicum annuum L.) SeedlingsPakistan Journal of Biological Sciences, 2011, 14(4), 300-304.
The Antioxidant Effect of Sacoglottis gabonensis Stem Bark Extract and Bergenin Isolate, Nigerian Alcoholic Beverage Additives on the Peroxidative Deterioration of Stored Vegetable OilsPakistan Journal of Biological Sciences, 2002, 5(5), 585-588.
Tocotrienol Rich Fraction Prevents Fenitrothion Induced Pancreatic Damage by Restoring Antioxidant StatusPakistan Journal of Biological Sciences, 2012, 15(11), 517-523.
Textile Effluent Induced Genotoxic Effects and Oxidative Stress in Clarias gariepinusPakistan Journal of Biological Sciences, 2012, 15(17), 804-812.
Some Properties of Inulinase from Aspergillus fumigatusPakistan Journal of Biological Sciences, 2002, 5(5), 589-593.
Allelopathic Potentials of Artrmisia monosperma and Thymus vulgaris on Growth and Physio-Biochemical Characteristics of Pea SeedlingsPakistan Journal of Biological Sciences, 2018, 21(4), 187-198.
PDGF-β and IL-18 Expressions on Carcinoma Cervical by Rhodomyrtus tomentosaPakistan Journal of Biological Sciences, 2022, 25(11), 986-992.
Comparison of Antioxidant Activity of Rheum ribes Fruits and Seed Methanolic Extracts against Protein Oxidation and Lipid PeroxidationPakistan Journal of Biological Sciences, 2015, 18(5), 232-239.
Chemopreventive and Antilipidperoxidative Efficacy of Annona squamosa Bark Extracts in Experimental Oral CarcinogenesisPakistan Journal of Biological Sciences, 2006, 9(14), 2600-2605.
Comparative Study of the Antioxidant Effect of Sacoglottis gabonensis Stem Bark Extract, a Nigerian Alcoholic Beverage Additive and Vitamins C and E on the Peroxidative Deterioration of some Common Stored Vegetable Oils in MaiduguriPakistan Journal of Biological Sciences, 2003, 6(3), 202-207.
Effects of Maghnian Bentonite on Physical Properties of Sandy Soils Under Semi-Arid Mediterranean ClimatePakistan Journal of Biological Sciences, 2008, 11(1), 17-25.
Antioxidant Responses of Two Barley Varieties to Saline StressPakistan Journal of Biological Sciences, 2008, 11(6), 905-909.
Effects of Salinity on Survival, Growth, Reproductive and Life Span Characteristics of Artemia Populations from Urmia Lake and Neighboring LagoonsPakistan Journal of Biological Sciences, 2008, 11(2), 164-172.
Effect of Salinity on Growth and Yield of Desi and Kabuli Chickpea CultivarsPakistan Journal of Biological Sciences, 2008, 11(4), 664-667.
Salinity Effects on Compatible Solutes, Antioxidants Enzymes and Ion Content in Three Wheat CultivarsPakistan Journal of Biological Sciences, 2008, 11(10), 1385-1389.
Growth and Some Physiological Parameters of Four Sugar Beet (Beta vulgaris L.) Cultivars as Affected by SalinityPakistan Journal of Biological Sciences, 2008, 11(10), 1390-1393.
Salt Stress Inhibits Germination and Early Seedling Growth in Cabbage (Brassica oleracea capitata L.)Pakistan Journal of Biological Sciences, 2007, 10(6), 910-914.
Chlorophylls, Proteins and Fatty Acids Amounts of Arthrospira Platensis Growing under Saline ConditionsPakistan Journal of Biological Sciences, 2007, 10(14), 2286-2291.
Larval Settlement of the Barnacle, Balanus improvisus Darwin (1854) under Different Food Concentration, Substratum, Light Period, Salinity, Cyprid Density and Cyprid AgePakistan Journal of Biological Sciences, 2007, 10(13), 2231-2236.
Effect of Salinity on Photosynthetic Efficiency and Yield of Rice GenotypesPakistan Journal of Biological Sciences, 1998, 1(2), 72-74.
Wheat Varietal Behavior in Hydroponic and Soil Culture Under Saline ConditionsPakistan Journal of Biological Sciences, 1998, 1(4), 318-320.
Effect of Seedling Age and Density on Growth and Yield of Rice in Saline SoilPakistan Journal of Biological Sciences, 1999, 2(3), 860-862.
Effects of Salinity and pH on Gas Exchange and Growth of Wheat under Hydroponic ConditionsPakistan Journal of Biological Sciences, 2000, 3(4), 644-646.
Yield and Yield Components of Pearl Millet as Affected by Various Salinity LevelsPakistan Journal of Biological Sciences, 2000, 3(9), 1393-1396.
Response of Various Sorghum Genotypes to Different Salinity Levels at Early Growth StagePakistan Journal of Biological Sciences, 2000, 3(9), 1406-1408.
Combined Effects of Temperature and Salinity Stress on Corn CV. SunahryPakistan Journal of Biological Sciences, 2000, 3(9), 1459-1463.
Study of Yield and Yield Components of Exotic Atriplex and Maireana Species in the Saline Environment of NWFPPakistan Journal of Biological Sciences, 2000, 3(11), 1873-1876.
Effect of Salt Stress on the Performance of Six Soybean GenotypesPakistan Journal of Biological Sciences, 2001, 4(2), 175-177.
Spatial Variability of Soil Salinity/sodicity and its Effect on Maize CropPakistan Journal of Biological Sciences, 2001, 4(2), 193-196.
Germination Potential of Chickpeas (Cicer arietinum L.) Under Saline ConditionsPakistan Journal of Biological Sciences, 2001, 4(4), 395-396.
Seasonal Nutrient Variations in Two Acacia Species Growing under Saline EnvironmentPakistan Journal of Biological Sciences, 2001, 4(5), 514-517.
Early Life History of Estuarine Grapsid Crab, Helice tridens latimeraPakistan Journal of Biological Sciences, 2001, 4(11), 1437-1439.
Differential Responses of Two Tomato Cultivars (Lycopersicon esculentum L.) to NaCl StressPakistan Journal of Biological Sciences, 2001, 4(12), 1456-1459.
Irrigation Water Quality Evaluation of Al-Hassa Springs and its Predictive Effects on Soil PropertiesPakistan Journal of Biological Sciences, 2002, 5(6), 651-655.
Factors Affecting the Efficiency of Symbiotic Nitrogen Fixation by RhizobiumPakistan Journal of Biological Sciences, 2002, 5(11), 1277-1293.
Performance of Intercropping of Maize with Groundnut in Saline Area under Rainfed ConditionPakistan Journal of Biological Sciences, 2003, 6(2), 92-94.
Changes in Quaternary Ammonium Compounds, Proline and Protein Profiles of Certain Halophytic Plants under Different Habitat ConditionsPakistan Journal of Biological Sciences, 2003, 6(10), 867-882.
Effect of NaCl and Na2SO4 Salinity on Leaf Appearance Stage, Tiller Production and Percent Motality of Spring WheatPakistan Journal of Biological Sciences, 2003, 6(24), 2001-2006.
Effect of Salinity on Some Yield Attributes of RicePakistan Journal of Biological Sciences, 2004, 7(5), 760-762.
Salinity on Cultivable Land and Its Effects on CropsPakistan Journal of Biological Sciences, 2004, 7(8), 1322-1326.
Germination and Protein patterns of some Genotypes of two species of Jute as affected by NaCl stressPakistan Journal of Biological Sciences, 2005, 8(2), 227-234.
Effect of Salinity on Ion Partitioning in Spring WheatPakistan Journal of Biological Sciences, 2005, 8(2), 302-306.
Physiological Characterization of Mustard (Brassica sp.) Genotypes for Their Salt TolerancePakistan Journal of Biological Sciences, 2005, 8(3), 433-438.
Effects of Endomycorrhizal Fungi and Salt Stress on Nutrient Acquisition and Growth of Pistacia vera L.Pakistan Journal of Biological Sciences, 2005, 8(7), 1006-1010.
Zinc and Salinity Interaction on Agronomical Traits, Chlorophyll and Proline Content in Lowland Rice (Oryza sativa L.) GenotypesPakistan Journal of Biological Sciences, 2006, 9(7), 1315-1319.
Responses of V. vinifera subsp. sylvestris (C.C. Gmelin) Ecotypes Originated from Two Different Geographical Regions of Turkey to Salinity Stress at Seed Germination and Plantlet StagesPakistan Journal of Biological Sciences, 2007, 10(16), 2631-2638.
Growth and Some Physiological Attributes of Pea (Pisum sativum L.) As Affected by SalinityPakistan Journal of Biological Sciences, 2007, 10(16), 2752-2755.
Effects of Salinity on Photosynthetic Pigments, Respiration, and Water Content in Two Barley VarietiesPakistan Journal of Biological Sciences, 2008, 11(20), 2438-2442.
Effect of Salinity on Organic Solutes Contents in BarleyPakistan Journal of Biological Sciences, 2009, 12(2), 158-162.
Seed Germination of Populations of Wild Wheat Species, Aegilops biuncialis and Ae. triuncialis: Effects of Salinity, Temperature and PhotoperiodPakistan Journal of Biological Sciences, 2006, 9(7), 1299-1305.
Effect of Sewage Irrigation on Nitrate Accumulation and Nitrate Reductase Activity in Leafy VegetablesPakistan Journal of Biological Sciences, 2012, 15(1), 34-38.
Effect of Azotobacter vinelandii and Compatible Solutes on Germination Wheat Seeds and Root Concentrations of Sodium and Potassium under Salt StressPakistan Journal of Biological Sciences, 2012, 15(3), 132-140.
RETRACTED: Study of Calendula and Gaillardia Growth in Two Composts Prepared from Agroindustrial WastesPakistan Journal of Biological Sciences, 2007, 10(9), 1438-1444.
Feasibility Study on the Culture of Mud Crab Scylla serrata in the Mid Coast Region of BangladeshPakistan Journal of Biological Sciences, 2012, 15(24), 1191-1195.
Screening Selected Genotypes of Cowpea [Vigna unguiculata (L.) Walp.] for Salt Tolerance During Seedling Growth StagePakistan Journal of Biological Sciences, 2013, 16(14), 671-679.
Spatial Distribution of Copepods along the Salinity Gradient of Perai River Estuary, Penang, MalaysiaPakistan Journal of Biological Sciences, 2012, 15(13), 647-652.
Effect of Water Salinity on the External Morphology of Ovarian Maturation Stages of Orange Mud Crab, Scylla olivacea (Herbst, 1796) in CaptivityPakistan Journal of Biological Sciences, 2016, 19(5), 219-226.
Ammonia Volatilization from Urea Applied to Salt-affected Soils under Flooded and Non-flooded ConditionsPakistan Journal of Biological Sciences, 1998, 1(4), 372-375.
Heterotic Effects in Spring Wheat (Triticum aestivum L.) Under Saline ConditionsPakistan Journal of Biological Sciences, 1998, 1(3), 159-162.
Combined Effect of Salinity and Hypoxia on Growth, Ionic Composition and Yield of Wheat Line 234-1Pakistan Journal of Biological Sciences, 1998, 1(3), 167-169.
Effects of Leaf Age on Ion Uptake in Wheat (Triticum aestivum L.) Under Saline ConditionsPakistan Journal of Biological Sciences, 1998, 1(3), 209-211.
Effect of salinity on germination and seedling growth of wheat (Triticum aestivum L.)Pakistan Journal of Biological Sciences, 1998, 1(3), 226-227.
Intravarietal Variability in Wheat Grown under Saline ConditionsPakistan Journal of Biological Sciences, 1999, 2(3), 693-696.
Effect of Salinity on the Chlorophyll Content, Yield and Yield Components of QPM CV. NutrictaPakistan Journal of Biological Sciences, 2000, 3(1), 43-46.
Influence of Sowing Methods on the Productivity of Canola Grown in Saline FieldPakistan Journal of Biological Sciences, 2000, 3(4), 687-691.
Response of Various Wheat Cultivars to Different Salinity Levels at Early Seedling StagePakistan Journal of Biological Sciences, 2000, 3(7), 1190-1193.
Studies of Pearl Millet under Salinity Stress at Early Growth StagePakistan Journal of Biological Sciences, 2000, 3(10), 1577-1579.
In vitro Survival and Nematicidal Activity of Rhizobium, Bradyrhizobium and Sinorhizobium. I. The Influence of Various NaCl ConcentrationsPakistan Journal of Biological Sciences, 2002, 5(6), 669-671.
Elevated CO2 and Stress Tolerance in Crop Plants with Particular Reference to Agro-climatic Conditions of PakistanPakistan Journal of Biological Sciences, 2003, 6(13), 1096-1107.
Effect of Iso-Osmotic Salt and Water Stress on Germination and Seedling Growth of Two Plantago speciesPakistan Journal of Biological Sciences, 2006, 9(15), 2812-2817.
Salinity and Preservation Conditions of Lipofuscin Extracts in Blue Swimming Crab, Portunus pelagicusPakistan Journal of Biological Sciences, 2020, 23(5), 685-690.
Effects of Boron and NaCl on Antioxidant Defence Mechanisms in Duckweeds (Spirodela polyrhiza L.)Pakistan Journal of Biological Sciences, 2021, 24(9), 989-996.
Seawater Quality Analysis in Mantehage Island for Integrated and Sustainable Marine Tourism DevelopmentPakistan Journal of Biological Sciences, 2021, 24(12), 1333-1339.
Optimum Processing Conditions for Bakasang Using the Response Surface Methodology with Central Composite Design (CCD)Pakistan Journal of Biological Sciences, 2021, 24(12), 1269-1277.
Response of Transgenic Rice at Germination and Early Seedling Growth Under Salt StressPakistan Journal of Biological Sciences, 2007, 10(23), 4303-4306.
Preliminary Assessment of ß-carotene Accumulation in Four Strains of Dunaliella salina Cultivated under the Different Salinities and Low Light IntensityPakistan Journal of Biological Sciences, 2006, 9(8), 1492-1496.
Effect of Salinity, Mineral Ions and Organic Solutes Contents on Vicia faba L.Pakistan Journal of Biological Sciences, 2006, 9(8), 1406-1410.
Effect of Salinity on in vitro Trichoderma harzianum Antagonism Against Verticillium dahliaePakistan Journal of Biological Sciences, 2005, 8(6), 872-876.
Effect of Alternating Saline and Non-saline Conditions on Emergence and Seedling Growth of RicePakistan Journal of Biological Sciences, 2004, 7(6), 883-890.
Multi-Objective Drainage Requirement of Harran PlainPakistan Journal of Biological Sciences, 2004, 7(2), 150-154.
Salt Stress Culture of Blue-green Algae SpirulinafusiformisPakistan Journal of Biological Sciences, 2003, 6(7), 648-650.
Comparative Efficiency of Foliar and Soil Application of K on Salt Tolerance in RicePakistan Journal of Biological Sciences, 2001, 4(7), 815-817.
Germination and Seedling Growth of Rice (Oryza sativa L.) Under Saline ConditionsPakistan Journal of Biological Sciences, 2000, 3(2), 350-351.
Effect of Different Salinity Levels on the Yield and Yield Components of Wheat CultivarsPakistan Journal of Biological Sciences, 2000, 3(7), 1161-1163.
Testing of Wheat (Triticum aestivum L.) Genotypes Against Salinity and WaterloggingPakistan Journal of Biological Sciences, 2000, 3(7), 1134-1137.
Investigating the Influence of Natural and Human Activities on Drinking Water Quality in Waterberg, South AfricaPakistan Journal of Biological Sciences, 2025, 28(3), 145-150.
Cited By
Genes on B chromosomes: Old questions revisited with new tools
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2015, 1849(1), 64. DOI: 10.1016/j.bbagrm.2014.11.007
Zika virus – emergence, evolution, pathology, diagnosis, and control: current global scenario and future perspectives – a comprehensive reviewVeterinary Quarterly, 2016, 36(3), 150. DOI: 10.1080/01652176.2016.1188333