Loop-mediated Isothermal Amplification of DNA (LAMP): A New Diagnostic Tool Lights the World of Diagnosis of Animal and Human Pathogens: A Review

Review Article
Loop-mediated Isothermal Amplification of DNA (LAMP): A New Diagnostic Tool Lights the World of Diagnosis of Animal and Human Pathogens: A Review

Kuldeep Dhama, K. Karthik, Sandip Chakraborty, Ruchi Tiwari, Sanjay Kapoor, Amit Kumar and Prasad Thomas

Pakistan Journal of Biological Sciences, 2014, 17(2), 151-166.


Diagnosis is an important part in case of animal husbandry as treatment of a disease depends on it. Advancement in molecular biology has generated various sophisticated tools like Polymerase Chain Reaction (PCR), its versions along with pen-side diagnostic techniques. Every diagnostic test however has both advantages and disadvantages; PCR is not an exception to this statement. To ease the odds faced by PCR several non-PCR techniques which can amplify DNA at a constant temperature has become the need of hour, thus generating a variety of isothermal amplification techniques including Nucleic Acid Sequence-Based Amplification (NASBA) along with Self-Sustained Sequence Replication (3SR) and Strand Displacement Amplification (SDA) and Loop mediated isothermal amplification (LAMP) test. LAMP stands out to be a good and effective diagnostic test for empowering in developing countries as it does not require sophisticated equipments and skilled personnel and proves to be cost-effective. Performance of LAMP mainly relies on crafting of six primers (including 2 loop primers) ultimately accelerating the reaction. LAMP amplifies DNA in the process pyrophosphates are formed causing turbidity that facilitates visualisation in a more effective way than PCR. The Bst and Bsm polymerase are the required enzymes for LAMP that does not possess 5'-3' exonuclease activity. Results can be visualized by adding DNA binding dye, SYBR green. LAMP is more stable than PCR and real-time PCR. Non-involvement of template DNA preparation and ability to generate 109 copies of DNA are added benefits that make it more effective than NASBA or 3SR and SDA. Thus, it fetches researcher’s interest in developing various versions of LAMP viz., its combination with lateral flow assay or micro LAMP and more recently lyophilized and electric (e) LAMP. Availability of ready to use LAMP kits has helped diagnosis of almost all pathogens. LAMP associated technologies however needs to be developed as a part of LAMP platform rather than developing them as separate entities. This review deals with all these salient features of this newly developed tool that has enlightened the world of diagnosis.

ASCI-ID: 1-6229

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The Egyptian Journal of Aquatic Research, 2019, 45(3), 231. DOI: 10.1016/j.ejar.2019.05.004

Assemblage of wetland bird Species in Purbasthali Oxbow Lake, West Bengal, India: Implications for Management

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Tolerance of pesticides and antibiotics among beneficial soil microbes recovered from contaminated rhizosphere of edible crops

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Preparation, urease inhibition mechanisms, and anti-Helicobacter pylori activities of hesperetin-7-rhamnoglucoside

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Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability

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Hypersaline environments as natural sources of microbes with potential applications in biotechnology: The case of solar evaporation systems to produce salt in Alicante County (Spain).

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Morphology, rheology and biodegradation of oxo-degradable polypropylene/polylactide blends

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Development and Evaluation of Loop-Mediated Isothermal Amplification (LAMP) as a Preliminary Diagnostic Tool for Brown Root Rot Disease Caused by Phellinus noxius (Corner) G. H. Cunningham in Hong Kong Urban Tree Management

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Preparation and incorporation of NiS[email protected] nano arrays in PVA matrix for resistive switching memory

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Experimental and theoretical study of the dye-sensitized solar cells using Hibiscus sabdariffa plant pigment coupled with polyaniline/graphite counter electrode

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Historical Westward Migration Phases of Ovis aries Inferred from the Population Structure and the Phylogeography of Occidental Mediterranean Native Sheep Breeds

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Phytochemicals and Antioxidant Activities of Conventionally Propagated Nodal Segment and In Vitro-Induced Callus of Bougainvillea glabra Choisy Using Different Solvents

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Dual Modulators of Selected Plant Secondary Metabolites Targeting COVID-19 Main Protease and Interleukin-2: An In-Silico Approach based Novel Hypothesis

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Does ISO 14001 and Green Servitization Provide a Push Factor for Sustainable Performance? A Study of Manufacturing Firms

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Transcriptional Comparison of Genes Associated with Photosynthesis, Photorespiration, and Photo-Assimilate Allocation and Metabolic Profiling of Rice Species

International Journal of Molecular Sciences, 2022, 23(16), 8901. DOI: 10.3390/ijms23168901

Groundwater status in Pakistan: A review of contamination, health risks, and potential needs

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Promising perspectives on novel protein food sources combining artificial intelligence and 3D food printing for food industry

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Impact of COVID-19 Pandemic on Animal Husbandry: A Review

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Corncob biocarriers with available carbon release for Chlamydopodium sp. microalgae towards enhanced nitrogen removal from low C/N rare earth element tailings (REEs) wastewater

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Paradigm shift from conventional processes to advanced membrane adsorption-mediated inactivation processes towards holistic management of virus − A critical review

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Selenium increases photosynthetic capacity, daidzein biosynthesis, nodulation and yield of peanuts plants (Arachis hypogaea L.)

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Efficacy of Levobupivacaine Versus Levobupivacaine Plus Dexmedetomidine Infiltration for Post-Tonsillectomy Analgesia: A Randomized Controlled Trial

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Experimental study on firebrick powder-based cementitious composites under the effect of elevated temperature

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Analysis of technical efficiency of dry season rice production in saline and non-saline areas of Bangladesh

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Deploying two-stage anaerobic process to co-digest greasy sludge and waste activated sludge for effective waste treatment and biogas recovery

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Recent advancements in the treatment of palm oil mill effluent (POME) using anaerobic biofilm reactors: Challenges and future perspectives

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Changes in the microbiota during biological treatment of palm oil mill waste: A critical review

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Academic Entrepreneurship: An Empirical Research of Invention Commercialisation

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Identification, molecular docking, and kinetic studies of six novel angiotensin-I-converting enzyme (ACE) inhibitory peptides derived from Kenaf (Hibiscus cannabinus L.) seed

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Development of a protocol for the elimination of Cyrtanthus elatus virus-A from Narcissus tazetta by in vitro chemotherapy in combination with electrotherapy

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Elimination of BBTV via a systemic in vitro electrotherapy approach

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Holothuria tubulosa as a bioindicator to analyse metal pollution on the coast of Alicante (Spain)

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Engineering of a core-shell polyvinyl alcohol/gelatin fibrous scaffold for dual delivery of Thymus daenensis essential oil and Glycyrrhiza glabra L. extract as an antibacterial and functional wound dressing

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Design and performance prediction of selective laser melted porous structure for femoral stem

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