Research Article
Experimental Pathological Studies of an Indian Chicken Anaemia Virus Isolate and its Detection by PCR and FAT

Mohd Yaqoob Wani, Kuldeep Dhama, Shyma K. Latheef, Rajamani Barathidassan, Ruchi Tiwari, Sandip Chakraborty, Milind Madhukar Chawak and Shambhu Dayal Singh

Pakistan Journal of Biological Sciences, 2014, 17(6), 802-811.

Abstract

Chicken Infectious Anaemia Virus (CIAV) is one of the potent immunosuppressive and economically important agents affecting poultry industry worldwide. Recent reports indicate the emergence of this virus in the poultry flocks of the country. The present study aimed to investigate the pathogenic potential of a recent isolate of CIAV obtained from poultry flock of Uttaranchal State, India. Twenty first day-old age Specific Pathogen Free (SPF) chicks were inoculated intramuscularly with 104.5 median tissue culture infective dose (TCID50) of CIAV passaged in the Marek’s disease virus transformed chicken splenic T lymphocyte (MDCC-MSB1) cell line while 15 chicks were kept as control. The CIAV isolate produced consistent clinical signs, loss in body weight gain, anaemia, low haematocrit values, bone marrow aplasia and generalized lymphoid atrophy. Mean Packed Cell Volume (PCV) value of the infected chicks was significantly low (18.22±2.22) compared to control group (34.12±4.72) at 14 day post infection (dpi). The establishment of virus infection in chicks was confirmed both at molecular and antigenic levels by Polymerase Chain Reaction (PCR) and Indirect Immunofluorescent Test (IIFT), respectively. Characteristic apoptotic pattern was also detected in the affected organs and the virus was re-isolated successfully in MDCC-MSB1 cell cultures. The present results revealed that the virus circulating in poultry flocks of Uttaranchal state is both pathogenic and immunosuppressive in nature. Extensive epidemiological studies are suggested in the poultry flocks of the country along with adaptation of appropriate diagnostic, prevention and control strategies so as to prevent economic losses caused by this important poultry pathogen.

ASCI-ID: 1-6416

Cited References Fulltext

Similar Articles


Cellular Death as a Regulative Mechanism in the Control of Cell-Number towards Normal Establishment of Orderly Structure and Function in the Petrous Ganglion during Development and Aging: An Investigation in the Chick

Pakistan Journal of Biological Sciences, 2000, 3(10), 1743-1748.

Evaluation of Apoptosis Induction Using PARP Cleavage on Gastric Adenocarcinoma and Fibroblast Cell Lines by Different Strains of Helicobacter pylori

Pakistan Journal of Biological Sciences, 2007, 10(22), 4097-4102.

A Study on the Effect of Thiotepa on Mice SpermatogenesisUsing Light and Electronic Microscope

Pakistan Journal of Biological Sciences, 2008, 11(15), 1929-1934.

Effects of Amodiaquine Hydrochloride and Artemisinin on Indomethacin-Induced Lipid Peroxidation in Rats

Pakistan Journal of Biological Sciences, 2008, 11(17), 2154-2158.

Dexamethasone Effects on Fas Ligand Expression in Mouse Testicular Germ Cells

Pakistan Journal of Biological Sciences, 2008, 11(18), 2231-2236.

Hepatitis C Infection and Apoptosis in Hepatocellular Carcinoma

Pakistan Journal of Biological Sciences, 2009, 12(10), 804-808.

Effect of Bone Morphogenetic Protein-2 on Normal and Osteoarthritic Human Articular Chondrocytes

Pakistan Journal of Biological Sciences, 2009, 12(17), 1194-1199.

Ultrastructural Alterations and Occurrence of Apoptosis in Developing Follicles Exposed to Low Frequency Electromagnetic Field in Rat Ovary

Pakistan Journal of Biological Sciences, 2007, 10(24), 4413-4419.

Dichloromethane-methanol Extract from Borassus aethiopum Mart. (Arecaceae) Induces Apoptosis of Human Colon Cancer Ht-29 Cells

Pakistan Journal of Biological Sciences, 2011, 14(10), 578-583.

Protective Effect of Berberine on Expression Pattern of Apoptotic, Cell Proliferative, Inflammatory and Angiogenic Markers During 7,12-dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis

Pakistan Journal of Biological Sciences, 2011, 14(20), 918-932.

Apoptosis-Sialic Acid Relationships at Different Developmental Stages of Murine Thymus

Pakistan Journal of Biological Sciences, 2006, 9(10), 1852-1860.

The Particularities of Protein fraction in the Apoptosis of Lymphocytes of Patients with Asthma

Pakistan Journal of Biological Sciences, 2013, 16(24), 1873-1883.

Alteration Expression of Bax, Bcl-2 and VDAC1 Genes in Oligozoospermic and Fertile Subjects

Pakistan Journal of Biological Sciences, 2016, 19(2), 71-76.

Anti-proliferation and Apoptosis Induction of Aqueous Leaf Extract of Carica papaya L. on Human Breast Cancer Cells MCF-7

Pakistan Journal of Biological Sciences, 2017, 20(1), 36-41.

Effects of Gold and Cerium Oxide Nanoparticles on Type 1 Diabetes in Experimental Mice

Pakistan Journal of Biological Sciences, 2020, 23(7), 959-967.

Pterygota alata (Roxb.) R.Br. Bark Fraction Induced Intrinsic Apoptotic Pathway in 4T1 Cells by Decreasing Bcl-2 and Inducing Bax Expression

Pakistan Journal of Biological Sciences, 2021, 24(2), 172-181.

Apoptotic Activity and Cytotoxic Effect of Convolvulus spicatus on Human Breast Cancer Cell Line (MCF-7)

Pakistan Journal of Biological Sciences, 2021, 24(6), 646-655.

Anticancer Activity of Pasak Bumi Root Extract (Eurycoma longifolia Jack) on Raji Cells

Pakistan Journal of Biological Sciences, 2021, 24(12), 1226-1235.

Effects of Oncogene Neuregulin 1 on Breast Cancer Cells

Pakistan Journal of Biological Sciences, 2022, 25(4), 345-352.

Analysis of Apoptotic Cells and Lung Inflammation after Given by Vitis gracilis

Pakistan Journal of Biological Sciences, 2022, 25(11), 1033-1039.

Time of Initial Detection of Fetal Structures and Anatomic Differentiation by Using B-Mode Ultrasound Examination in Bitches

Pakistan Journal of Biological Sciences, 2008, 11(13), 1750-1753.

Comparison of ELISA and Microscopy for Detection of Cryptosporidium Oocysts in Animals

Pakistan Journal of Biological Sciences, 2015, 18(7), 341-345.

Study the Effects of Different Levels of Fat and L-carnitine on Performance, Carcass Characteristics and Serum Composition of Broiler Chicks

Pakistan Journal of Biological Sciences, 2007, 10(12), 1970-1976.

Immunomodulators in Day to Day Life: A Review

Pakistan Journal of Biological Sciences, 2013, 16(17), 826-843.

Isolation of Bovine Coronavirus (BCoV) in Vero Cell Line and its Confirmation by Direct FAT and RT-PCR

Pakistan Journal of Biological Sciences, 2013, 16(21), 1342-1347.

Egg Drop Syndrome-76 (EDS-76) in Japanese Quails (Coturnix coturnix japonica): An Experimental Study Revealing Pathology, Effect on Egg Production/Quality and Immune Responses

Pakistan Journal of Biological Sciences, 2014, 17(6), 821-828.

The Influence of Galium aparine L. Seeds on the Health Status of Broiler Chickens

Pakistan Journal of Biological Sciences, 2000, 3(5), 785-786.

Hepatoprotective Role of Clay and Nano Clay for Alleviating Aflatoxin Toxicity in Male Rats

Pakistan Journal of Biological Sciences, 2021, 24(10), 1091-1102.

Enumeration of Lactobacilli in the Fecal Flora of Infant Using Two Different Modified de-Man Rogosa Sharpe Media under Aerobic and Anaerobic Incubation

Pakistan Journal of Biological Sciences, 2008, 11(6), 876-881.

Isolation and Identification of Ornithobacterium rhinotracheale in Broiler Breeder Flocks of Guilan Province, North of Iran

Pakistan Journal of Biological Sciences, 2008, 11(11), 1487-1491.

Isolation and Characterization of Campylobacter spp. from Domestic Animals and Poultry in South of Iran

Pakistan Journal of Biological Sciences, 2007, 10(9), 1519-1524.

Effect of Storage Temperature on the Quality and Quantity of DNA Extracted from Blood

Pakistan Journal of Biological Sciences, 2000, 3(3), 392-394.

Isolation and Characterization of Bacterial Species from Surgical and Non-surgical Wounds Located on Body Surface of Buffaloes, Cattles, Sheep and Goats

Pakistan Journal of Biological Sciences, 2001, 4(6), 696-702.

The β-galactosidase System of a Novel Plant from Durian Seeds (Durio zibethinus) I. Isolation and Partial Characterization

Pakistan Journal of Biological Sciences, 2001, 4(12), 1531-1534.

Entomopathogenic Nematodes Associated with Soil Types and Vegetation Cover in Pothwar Region of Pakistan

Pakistan Journal of Biological Sciences, 2002, 5(6), 640-642.

Cultural Sensitivity of Septic Wound in Animals

Pakistan Journal of Biological Sciences, 2003, 6(8), 741-744.

Isolation, Purification and Characterization of Biodegradable Polymer Producing Bacteria Listeria murrayi

Pakistan Journal of Biological Sciences, 2004, 7(11), 2018-2021.

Isolation and Detection of MTBE Degrading Bacteria

Pakistan Journal of Biological Sciences, 2005, 8(7), 974-977.

Isolation of Saponin from Dried Roots of Gypsophila simonii Hub. Mor

Pakistan Journal of Biological Sciences, 2007, 10(11), 1944-1946.

6-Aminopenicillanic Acid Production by Intact Cells of E. coli Containing Penicillin G Acylase (PGA)

Pakistan Journal of Biological Sciences, 2007, 10(18), 3190-3194.

Isolation and Characterization of BoHV-1 from Seropositive Cows after Inducing Artificial Stress in West Bengal, India

Pakistan Journal of Biological Sciences, 2013, 16(15), 720-725.

Bioassay-guided Isolation and Identification of Antioxidative Compounds from the Bark of Eugenia polyantha

Pakistan Journal of Biological Sciences, 2013, 16(16), 812-818.

Isolation of Bovine Coronavirus (BCoV) in Vero Cell Line and its Confirmation by Direct FAT and RT-PCR

Pakistan Journal of Biological Sciences, 2013, 16(21), 1342-1347.

Isolation of GSH from Baker Yeast by Homogenizer Method

Pakistan Journal of Biological Sciences, 2004, 7(9), 1636-1639.

Isolation of Toxoplasma gondii from Horse Meat in Egypt

Pakistan Journal of Biological Sciences, 2007, 10(1), 174-177.

Isolation and Characterization of Antagonistic Activity of Some Streptomycetes and their Specific Actinophages from Soil

Pakistan Journal of Biological Sciences, 2021, 24(4), 516-526.

Importance of Seed-Borne Fungi of Sorghum and Pearl Millet in Burkina Faso and Their Control Using Plant Extracts

Pakistan Journal of Biological Sciences, 2008, 11(3), 321-331.

Determination of the Anastomosis Grouping and Virulence of Rhizoctonia solani Kuhn Isolates Associated with Bean Plants Grown in Samsun/Turkey

Pakistan Journal of Biological Sciences, 2002, 5(4), 434-437.

Biocontrol of Sugarbeet Pathogen Fusarium solani (Mart.) Sacc. by Streptomyces aureofaciens

Pakistan Journal of Biological Sciences, 2002, 5(5), 556-559.

Pathogenicity and Antimicrobial Activity of Seed-before Fusarium solani (Mart.) Appel and Wollenw. Emend. Snyd and Hans Strains

Pakistan Journal of Biological Sciences, 2003, 6(13), 1183-1186.

Study on the Isolation and Pathogenicity of Pasteurella multocida Type A in Calves in Saudi Arabia

Pakistan Journal of Biological Sciences, 2004, 7(4), 460-463.

Toxicity/Pathogenicity Evaluation of Metarhizium anisopliae LMA-06 by Means of Oral and Intranasal Dosing

Pakistan Journal of Biological Sciences, 2005, 8(7), 969-973.

Determination of the Anastomosis Grouping and Virulence of Rhizoctonia spp. Associated with Potato Tubers Grown in Lincoln, New Zealand

Pakistan Journal of Biological Sciences, 2007, 10(21), 3786-3793.

Pathogenicity of Fusarium isolates to Striga hermonthica in Burkina Faso

Pakistan Journal of Biological Sciences, 2010, 13(5), 201-208.

Occurrence, Pathogenicity and Distribution of Fusarium spp. in Stored Wheat Seeds Kermanshah Province, Iran

Pakistan Journal of Biological Sciences, 2010, 13(24), 1178-1186.

Respiratory Bacterial Flora from Healthy as well as Respiratory Symptoms’ Subjects

Pakistan Journal of Biological Sciences, 2011, 14(7), 456-460.

Pathogenicity of Field Isolates of Newcastle Disease Virus

Pakistan Journal of Biological Sciences, 2000, 3(6), 1083-1085.

Seed-Borne Fungi of Peanut in Egypt: Pathogenicity and Transmission

Pakistan Journal of Biological Sciences, 2001, 4(1), 63-68.

Prevalence and Pathogenicity of Guava Anthracnose with Special Emphasis on Varietal Reaction

Pakistan Journal of Biological Sciences, 2003, 6(3), 234-241.

Identification and Pathogenicity of Fungal Dieback Disease on Sengon (Paraserianthes falcataria (L.) Nielsen) Seedling and Rice (Oryza sativa)

Pakistan Journal of Biological Sciences, 2018, 21(1), 16-23.

Substantial Virulence Genes among Brucella melitensis Field Strains Isolated from Cattle in Egypt

Pakistan Journal of Biological Sciences, 2019, 22(5), 239-246.

Inhibitory Effect of Aqueous and Ethanolic Extracts of Neem Parts on Fungal Rot Disease of Solanum tuberosum

Pakistan Journal of Biological Sciences, 2019, 22(5), 206-213.

Treatment Trial of Nile Tilapia (Oreochromis niloticus) Experimentally Infected with Vibrio alginolyticus Isolated from Sea bass (Dicentrarchus labrax)

Pakistan Journal of Biological Sciences, 2020, 23(12), 1591-1600.

Rapid Detection of Salmonella enteritidis by PCR Amplification of The SefA Gene and It`s Cloning

Pakistan Journal of Biological Sciences, 2008, 11(3), 428-432.

Molecular Identification of Streptococcus equi subsp. Equi and Streptococcus equi subsp. zooepidemicus in Nasal Swabs Samples from Horses Suffering Respiratory Infections in Iran

Pakistan Journal of Biological Sciences, 2008, 11(3), 468-471.

Detection and Frequency of Stx2 Gene in Escherichia coli O157 and O157:H7 Strains Isolated from Sheep Carcasses in Shiraz-Iran

Pakistan Journal of Biological Sciences, 2008, 11(8), 1085-1092.

Separation of DNA for Molecular Markers Analysis from Leaves of the Vitis vinifera

Pakistan Journal of Biological Sciences, 2008, 11(11), 1436-1442.

Assessment of Chronic Gastritis in Pet Dogs and its Relation with Helicobacter-Like Organisms

Pakistan Journal of Biological Sciences, 2008, 11(11), 1443-1448.

Monitoring of Brucella Reactor does Following Milk Examination Using Different Techniques

Pakistan Journal of Biological Sciences, 2007, 10(2), 240-244.

Evaluation of Different Primer Sets for the Rapid Diagnosis of Tuberculosis

Pakistan Journal of Biological Sciences, 2007, 10(1), 107-111.

A Simple and Rapid Assay for Specific Identification of Bovine Derived Products in Biocomplex Materials

Pakistan Journal of Biological Sciences, 2007, 10(8), 1170-1174.

Rapid Detection of Salmonella dublin by PCR Amplification of the SopE Gene and its Cloning

Pakistan Journal of Biological Sciences, 2008, 11(11), 1497-1501.

Amplification and Cloning of Herpes Simplex Virus Type 2 Glycoprotein G from an Iranian Isolate

Pakistan Journal of Biological Sciences, 2007, 10(6), 955-958.

Entomocidal Activity of Bacillus Thuringiensis Transgenic Rice Plants Against Rice Leaffolder, Cnaphalocrocis Medinalis (Lepidoptera: Pyralidae).

Pakistan Journal of Biological Sciences, 1999, 2(4), 1472-1477.

PCR Amplification and Cloning of T-DNA Homologous Sequences from Nicotiana glauca

Pakistan Journal of Biological Sciences, 2002, 5(7), 780-783.

Coagulase Gene Polymorphism of Staphylococcus Aureus Strains Isolated from Human, Animals and Environment

Pakistan Journal of Biological Sciences, 2005, 8(2), 278-280.

Rapid Detection of Pork in Processed Food Using Polymerase Chain Reaction Amplification Technology: A Preliminary Report

Pakistan Journal of Biological Sciences, 2005, 8(3), 501-504.

Prevalence and Incidence of Bacterial Spot Disease Caused by Xanthomonas campestris pv. vesicatoria on Pepper in the Eastern Mediterranean Region of Turkey

Pakistan Journal of Biological Sciences, 2005, 8(12), 1656-1658.

Phlebotpmus (Larroussius) kandelakii the Principal and Proven Vector of Visceral Leishmaniasis in North West of Iran

Pakistan Journal of Biological Sciences, 2005, 8(12), 1802-1806.

A Comparison Between Cerebrospinal Fluid Pretreatment Protocols Prior to Diagnosis of Herpes Simplex Encephalitis by PCR

Pakistan Journal of Biological Sciences, 2006, 9(10), 1884-1890.

Genetic Differentiation and Phylogenetic Relationships Among Barbus xanthopterus (Cyprinidae) Populations in Southwest of Iran Using Mitochondrial DNA Markers

Pakistan Journal of Biological Sciences, 2006, 9(12), 2249-2254.

Molecular Study of Salmonella enteritidis in Poultry Samples by PCR, Plasmid Curing, Antibiotic Resistance and Protein Pattern Analysis

Pakistan Journal of Biological Sciences, 2007, 10(10), 1562-1570.

The Efficiency of ELISA and PCR in Detecting Subclinical Paratuberculosis in the Saudi Dairy Herds

Pakistan Journal of Biological Sciences, 2007, 10(11), 1906-1909.

PCR Based on Identification of Vectors of Zoonotic Cutaneous Leishmaniasis in Shahrood District, Central of Iran

Pakistan Journal of Biological Sciences, 2007, 10(12), 2061-2065.

Detection of Ureaplasma urealyticum in Semen of Infertile Men by PCR

Pakistan Journal of Biological Sciences, 2007, 10(21), 3960-3963.

Vancomycin-Resistant Enterococcus faecalis from a Wastewater Treatment Plant in Tabriz, Iran

Pakistan Journal of Biological Sciences, 2008, 11(20), 2443-2446. DOI:

Designing and Introduce a Diagnostic Kit for Detection of White Spot Syndrome Virus in Cultured Penaeus indicus in Iran

Pakistan Journal of Biological Sciences, 2008, 11(24), 2660-2664.

The First Report of SVC from Indian Carp Species by PCR and Histopathologic Methods in Iran

Pakistan Journal of Biological Sciences, 2008, 11(24), 2675-2678.

Identification of a Novel Missense Mutation in Exon 4 of the Human Factor VIII Gene Associated with Sever Hemophilia a Patient

Pakistan Journal of Biological Sciences, 2007, 10(23), 4299-4302.

Molecular G Typing of Bovine Rotaviruses in Iran

Pakistan Journal of Biological Sciences, 2007, 10(19), 3466-3469.

Chlamydia trachomatis Prevalence in Iranian Women Attending Obstetrics and Gynaecology Clinics

Pakistan Journal of Biological Sciences, 2007, 10(24), 4490-4494.

Rapid Detection of Borrelia burgdorferi Strains by Nested Polymerase Chain Reaction

Pakistan Journal of Biological Sciences, 2009, 12(5), 463-466.

Intraspecific Genetic Variation in the Japanese Loach (Misgurnus anguillicaudatus) Revealed by Random Amplified Polymorphic DNA (RAPD) Analysis

Pakistan Journal of Biological Sciences, 2001, 4(7), 877-880.

Fowl Adenovirus (FAdV) in India: Evidence for Emerging Role as Primary Respiratory Pathogen in Chickens

Pakistan Journal of Biological Sciences, 2012, 15(18), 900-903.

Zoonotic Chicken Toxoplasmosis in Some Egyptians Governorates

Pakistan Journal of Biological Sciences, 2012, 15(17), 821-826.

Comparison of PCR, Wright Agglutination Test and Blood Culture for Diagnosis of Brucellosis in Suspected Patients

Pakistan Journal of Biological Sciences, 2013, 16(22), 1589-1592.

Transformation of Morinda citrifolia Via Simple Mature Seed Imbibition Method

Pakistan Journal of Biological Sciences, 2013, 16(24), 1913-1921.

Rapid Identification of Enterovirulent Escherichia coli Strains using Polymerase Chain Reaction from Shrimp Farms

Pakistan Journal of Biological Sciences, 2013, 16(21), 1260-1269.

A Study on the Wool Characteristics of Several Turkish Sheep Breeds According to the Microsatellite DNA Types

Pakistan Journal of Biological Sciences, 2005, 8(2), 186-189.

Application of Molecular and Serological Methods for Rapid Detection of Mycoplasma gallisepticum Infection (Avian mycoplasmosis)

Pakistan Journal of Biological Sciences, 2015, 18(2), 81-87.

Rice Tungro Disease in Sarawak: Past and Present Status

Pakistan Journal of Biological Sciences, 2015, 18(6), 285-289.

Polymerase Chain Reaction-based Detection of Cotton Leaf Curl and Other Whitefly-transmitted Geminiviruses from Sindh

Pakistan Journal of Biological Sciences, 1998, 1(1), 39-43.

Effect of Combination Chemotherapy on Hepatitis C Virus in Hepatic Patients

Pakistan Journal of Biological Sciences, 2000, 3(6), 969-970.

Detection of Toxoplasma gondii from Sera and Urine of Experimentally Infected Mice by PCR

Pakistan Journal of Biological Sciences, 2007, 10(1), 193-195.

Molecular Identification and Sequencing of Mycoplasma gallisepticum Recovered from Broilers in Egypt

Pakistan Journal of Biological Sciences, 2018, 21(5), 253-261.

Substantial Virulence Genes among Brucella melitensis Field Strains Isolated from Cattle in Egypt

Pakistan Journal of Biological Sciences, 2019, 22(5), 239-246.

Toxoplasma gondii Infection in Cattle Egret (Bubulcus ibis): First Report from Shebin El-Kom, Menoufia Governorate, Egypt

Pakistan Journal of Biological Sciences, 2020, 23(11), 1442-1449.

Immunological, Histopathological, Molecular Identification and Genotyping of Toxoplasma gondii in Small Ruminants in Egypt

Pakistan Journal of Biological Sciences, 2022, 25(2), 144-153.

Molecular Identification of Phytophthora sp. From Indonesian Cocoa Using Phylogenetic Analysis

Pakistan Journal of Biological Sciences, 2022, 25(3), 245-253.

Molecular Identification of Lobster Species Based on Cytochrome Oxidase Subunit I Gene characters

Pakistan Journal of Biological Sciences, 2022, 25(6), 501-508.

Cited By


Molecular characterization of chicken anemia virus outbreaks in Nagpur province, India from 2012 to 2015

Microbial Pathogenesis, 2017, 102(), 113. DOI: 10.1016/j.micpath.2016.11.017