Cellulase Production by Fusarium graminearum and its Application in Ruminant’s Diets Degradation

Research Article
Cellulase Production by Fusarium graminearum and its Application in Ruminant’s Diets Degradation

H.H. Azzaz, H.A. Murad, A.A. Aboamer, Hoda Alzahar and M. Fahmy

Pakistan Journal of Biological Sciences, 2020, 23(1), 27-34.


Background and Objective: Cellulase as a fibrolytic enzyme is a highly effective tool for agricultural waste treatments. Production of cellulase enzyme on medium of agricultural wastes by Fusarium graminearum to be used in ruminant feeding was the main objective of this study. Materials and Methods: Impact of initial pH of growth medium, different nitrogen sources and variety of agriculture by products as a carbon sources on cellulase production have been studied. Electron microscope was used for investigate the impact of the resultant cellulase on corn stover degradation, while batch culture technique was used for investigate impact of different levels of the produced and commercial cellulases on total mixed ration digestibility by rumen microorganisms (in vitro). Results: Cellulase maximum production by F. graminearum was obtained at 20% corn stover, initial pH of growth medium 5.0 and peptone as a nitrogen source. All addition levels of the produced cellulase increased dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose and hemicellulose degradability of the treated diets, but the maximum produced cellulase efficiency% for dry matter degradability was obtained at 1200 IU kg1 DM reached 23.19% over the control. Conclusion: Utilization of the produced cellulase in enrichment of the feeding value of the agricultural by-products may help in overcome of the feed gap with good impact on environment and public health.

ASCI-ID: 1-6788

Cited References Fulltext

Cited By

Fusarium graminearum as a producer of xylanases with low cellulases when grown on wheat bran

Biotechnology Reports, 2022, (), e00738. DOI: 10.1016/j.btre.2022.e00738

Effect of HAc on the Metastable Pitting Corrosion of 304 SS in NaCl Solution

Materials, 2022, 15(10), 3618. DOI: 10.3390/ma15103618

Metal–organic framework on porous TiO2 thin film-coated alumina beads for fractional distillation of plant essential oils

Analytical and Bioanalytical Chemistry, 2022, (), . DOI: 10.1007/s00216-022-04103-6

Changes in the distribution and pest risk of stored product insects in Europe due to global warming: Need for pan-European pest monitoring and improved food-safety

Journal of Stored Products Research, 2022, 97(), 101977. DOI: 10.1016/j.jspr.2022.101977

Implementation of 5S in a plastic bag manufacturing industry: A case study

Cleaner Engineering and Technology, 2022, 8(), 100488. DOI: 10.1016/j.clet.2022.100488

Removal and Recovery of an Anionic Surfactant in the Presence of Alcohol by Foam Fractionation

Industrial & Engineering Chemistry Research, 2022, (), . DOI: 10.1021/acs.iecr.2c00372

Derivatization-free multi-step extraction strategy for trace haloacetic acids analysis with ion chromatography: Performance and mechanisms

Journal of Hazardous Materials, 2022, (), 129166. DOI: 10.1016/j.jhazmat.2022.129166

Assessment of the impact of anthropogenic stress on wetlands of the upper Ganga Delta, India: a multi criteria decision making approach

Geocarto International, 2022, (), 1. DOI: 10.1080/10106049.2022.2068678

Molecular approaches reveal speciation between red- and blue-flowered plants in the Mediterranean Lysimachia arvensis and L. monelli (Primulaceae)

Botanical Journal of the Linnean Society, 2022, 199(2), 557. DOI: 10.1093/botlinnean/boab081

Recent demulsification methods of crude oil emulsions – Brief review

Journal of Petroleum Science and Engineering, 2022, (), 110643. DOI: 10.1016/j.petrol.2022.110643