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Research Article
An Efficient Micropropagation System for Vitex negundo L., an Important Woody Aromatic Medicinal Plant, Through Shoot Tip Culture

P.K. Usha, Sailas Benjamin, K.V. Mohanan and A.V. Raghu

Research Journal of Botany, 2007, 2(2), 102-107.


An efficient protocol was established for in vitro shoot multiplication from shoot tip explants of Vitex negundo on Murashige and Skoog (MS) basal medium supplemented with 8.87 μM 6-benzylaminopurine (BA). Inclusion of 8-Naphthalene Acetic Acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate shoot multiplication (6.3) after 4 week of culture on MS basal medium supplemented with 8.87 μM BA, 2.69 μM NAA. The elongated shoots rooted in half strength MS basal salts supplemented with 4.90 μM IBA + 2.85 μm IAA and 2% (w/v) sucrose. The presence of Activated Charcoal (AC) with IBA showed positive response to rooting. In vitro propagated plants were transferred to soil with a survival rate of 85% after 1 month.

ASCI-ID: 60-34

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