The Influence of Exogenous Enzyme, Formaldehyde and/or Sodium Hydroxide on in vitro Gas Production Parameters of Sunflower Meal

Research Article
The Influence of Exogenous Enzyme, Formaldehyde and/or Sodium Hydroxide on in vitro Gas Production Parameters of Sunflower Meal

M. Danesh Mesgaran, T. Mohammadabadi, M. Chaji and M.R. Ghorbani

Research Journal of Biological Sciences, 2009, 4(9), 1068-1072.

Abstract

This experiment was conducted to evaluate the effect of exogenous enzyme (3 g kg^-1 DM), formaldehyde (30 and 60 g kg^-1 DM) and or sodium hydroxide (40 g kg^-1 DM) on in vitro gas production parameters of sunflower meals (25 and 165 g fat kg^-1 DM) were with gas production technique and using fistulated sheep rumen fluid. Kinetics of gas production was fitted to an exponential model. Sodium hydroxide and enzyme treatments significantly increased gas production (B), Metabolizable Energy (ME), Organic Matter Digestibility (OMD), Net Energy Lactation (NEL), ammonia-N (NH₃-N) and Short Chain Fatty Acid (SCFA) but formaldehyde decreased them. The lowest of gas production was estimated for low fat sunflower meal treated with 60 g formaldehyde/kg DM (75.9 mL/500 mg DM). There was significant difference among samples for gas production rate Constant (C). Sodium hydroxide treated high fat sunflower meal had the highest of ME, OMD, NEL and SCFA (35.8 MJ kg^-1 DM, 207.5 g kg^-1 OM, 2.42 Mcal kg^-1 DM and 1.53 μmol L^-1, respectively). Ammonia-N concentration of high fat sunflower meal were the highest (40.9 mg/100 mL). The values of B, ME, OMD, NEL, NH₃-N and SCFA for low fat sunflower meal was less than high fat sunflower meal. The results showed, it may be that gas production parameters of low fat and high fat sunflower meal are influenced by exogenous enzyme, formaldehyde and NaOH.