Research Article 
Oxidative Damage and Reproductive Toxicity Associated with Cyromazine and
Chlorpyrifos in Male Rats: The Protective Effects of Green Tea Extract
Tarek M. Heikal, Abdel-Tawab H. Mossa, Azza W. Ibrahim and Hala F. Abdel-Hamid
Research Journal of Environmental Toxicology, 2014, 8(2), 53-67.
Abstract
Pesticides have contributed for many public health hazards in man including infertility. So, the present study aimed to assess the protective role of green tea extract (GT) against the possibility of reproductive toxicity resulting from chlorpyrifos (CPF), cyromazine (Cyr) and their combination exposure in mature male Wistar rats. Rats were administered CPF (5.4 mg kg-1 b.wt., 1/25 LD50), Cyr (135.48 mg kg-1 b.wt., 1/25 LD50), CPF+Cyr, GT (1.5% w/v in water) as the only drinking fluid, CPF+GT, Cyr+GT and CPF+Cyr+GT daily via gavage for 70 days to complete the spermatogenic cycle. The results revealed that exposure to CPF, Cyr and CPF+Cyr significantly decreased the fertility index, weights of sexual organs (testes, seminal vesicles, epidermis and prostate gland), sperm characteristics (motility and count) as well as serum testosterone level, while increased sperm abnormality. In addition, the testicular tissue level of reduced glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) enzymes were significantly decreased while increased the level of testicular tissue lipid peroxidation (LPO) compared with the control group. The testicular histopathological lesions were characterized by moderate to severe degenerative changes of seminiferous tubules and incomplete arrest of spermatogenesis. Co-administration of GT to treated-animals alleviates the reproductive toxicity and testicular oxidative damage. In conclusion, the use of green tea extract appeared to be beneficial in attenuating and improving the testicular damage and reproductive toxicity sustained by insecticide exposure in male rats.
ASCI-ID: 71-171
Related Articles
Chlorpyrifos-Induced Alteration of Hematological Parameters in Wistar Rats: Ameliorative Effect of Zinc
Research Journal of Environmental Toxicology, 2010, 4(2), 55-66.
Detrimental Effects of Chlorpyrifos and Cypermethrin on Reproductive Physiology of Male Albino RatsResearch Journal of Environmental Toxicology, 2016, 10(1), 68-74.
Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian MenResearch Journal of Environmental Toxicology, 2011, 5(5), 282-292.
Cited By
Individual and combined effect of chlorpyrifos and cypermethrin on reproductive system of adult male albino rats
Environmental Science and Pollution Research, 2017, 24(2), 1532. DOI: 10.1007/s11356-016-7912-6
Low doses of chlorpyrifos interfere with spermatogenesis of rats through reduction of sex hormonesEnvironmental Science and Pollution Research, 2017, (), . DOI: 10.1007/s11356-017-9617-x
Fluorometric and molecular modeling deciphering the non-covalent interaction between cyromazine and human serum albuminJournal of Inclusion Phenomena and Macrocyclic Chemistry, 2017, (), . DOI: 10.1007/s10847-017-0754-0
Beneficial effects of folic acid on the kidneys and testes of adult albino rats after exposure to methomylToxicology Research, 2018, (), . DOI: 10.1039/C7TX00309A
Ameliorative effect of nanoencapsulated flavonoid against chlorpyrifos-induced hepatic oxidative damage and immunotoxicity in Wistar ratsJournal of Biochemical and Molecular Toxicology, 2018, (), e22050. DOI: 10.1002/jbt.22050
In vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo developmentEnvironmental and Molecular Mutagenesis, 2018, (), . DOI: 10.1002/em.22242
Chlorpyrifos-induced reproductive toxicity in rats could be partly relieved under high-fat dietChemosphere, 2019, 229(), 94. DOI: 10.1016/j.chemosphere.2019.05.020
Chlorpyrifos induces metabolic disruption by altering levels of reproductive hormonesJournal of Agricultural and Food Chemistry, 2019, (), . DOI: 10.1021/acs.jafc.9b03602
Iprodione and/or chlorpyrifos exposure induced testicular toxicity in adult rats by suppression of steroidogenic genes and SIRT1/TERT/PGC-1α pathwayEnvironmental Science and Pollution Research, 2021, (), . DOI: 10.1007/s11356-021-14339-x
Iprodione and chlorpyrifos induce testicular damage, oxidative stress, apoptosis and suppression of steroidogenic‐ and spermatogenic‐related genes in immature male albino ratsAndrologia, 2021, (), . DOI: 10.1111/and.13978
Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administrationChemico-Biological Interactions, 2021, 333(), 109312. DOI: 10.1016/j.cbi.2020.109312
Testicular deficiency associated with exposure to cypermethrin, imidacloprid, and chlorpyrifos in adult ratsEnvironmental Toxicology and Pharmacology, 2021, (), 103724. DOI: 10.1016/j.etap.2021.103724
Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male ratsToxicological Research, 2022, (), . DOI: 10.1007/s43188-021-00120-2
Injurious Effects of Glyphosate on Sperm Profile and Testicular Tissues of Wistar RatAsian Journal of Applied Sciences, 2021, 14(1), 9. DOI: 10.3923/ajaps.2021.9.14