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Research Article
Oxidative Damage and Reproductive Toxicity Associated with Cyromazine and Chlorpyrifos in Male Rats: The Protective Effects of Green Tea Extract

Tarek M. Heikal, Abdel-Tawab H. Mossa, Azza W. Ibrahim and Hala F. Abdel-Hamid

Research Journal of Environmental Toxicology, 2014, 8(2), 53-67.

Abstract

Pesticides have contributed for many public health hazards in man including infertility. So, the present study aimed to assess the protective role of green tea extract (GT) against the possibility of reproductive toxicity resulting from chlorpyrifos (CPF), cyromazine (Cyr) and their combination exposure in mature male Wistar rats. Rats were administered CPF (5.4 mg kg-1 b.wt., 1/25 LD50), Cyr (135.48 mg kg-1 b.wt., 1/25 LD50), CPF+Cyr, GT (1.5% w/v in water) as the only drinking fluid, CPF+GT, Cyr+GT and CPF+Cyr+GT daily via gavage for 70 days to complete the spermatogenic cycle. The results revealed that exposure to CPF, Cyr and CPF+Cyr significantly decreased the fertility index, weights of sexual organs (testes, seminal vesicles, epidermis and prostate gland), sperm characteristics (motility and count) as well as serum testosterone level, while increased sperm abnormality. In addition, the testicular tissue level of reduced glutathione (GSH) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) enzymes were significantly decreased while increased the level of testicular tissue lipid peroxidation (LPO) compared with the control group. The testicular histopathological lesions were characterized by moderate to severe degenerative changes of seminiferous tubules and incomplete arrest of spermatogenesis. Co-administration of GT to treated-animals alleviates the reproductive toxicity and testicular oxidative damage. In conclusion, the use of green tea extract appeared to be beneficial in attenuating and improving the testicular damage and reproductive toxicity sustained by insecticide exposure in male rats.

ASCI-ID: 71-171

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