Research Article
Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase^® Enzyme: A Response Surface Approach

Herpandi, N. Huda, A. Rosma and W.A. Wan Nadiah

Journal of Fisheries and Aquatic Science, 2013, 8(4), 494-505.

Abstract

The goal of this study was to optimize enzymatic hydrolysis of the dark flesh of skipjack tuna (Katsuwonus pelamis). Alcalase 2.4 L^® was used as the hydrolytic enzyme at various concentrations (1, 1.5, 2, 2.5 and 3% w/w) at pHs from 6 to 10, temperatures from 35 to 75°C and times of 2 to 6 h. The experiment was performed following a five-level second-order central composite design with six replications at the center points. The high coefficients of determination for degree of hydrolysis (R² = 0.8909) and free tryptophan content (R² = 0.9732) indicate the suitability of the design for predicting the responses. The optimum hydrolytic conditions predicted by the response surface methodology were 2% Alcalase^® at pH 8.86 and 65.4°C for 5.74 h. These conditions resulted in an experimental degree of hydrolysis of 20.74% and free tryptophan content of 102.23 mg kg^-1.

ASCI-ID: 54-441

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PeerJ, 2019, 7(), e8297. DOI: 10.7717/peerj.8297