Identification of Callus Induction and Plant Regeneration Responsiveness in Presence of NaCl in in vitro Culture of Some Deepwater Rice (Oryza sativa L.) Cultivars

Research Article
Identification of Callus Induction and Plant Regeneration Responsiveness in Presence of NaCl in in vitro Culture of Some Deepwater Rice (Oryza sativa L.) Cultivars

L. Khaleda, A.M.A. Ahmed, L.W. Marzan and M. Al-Forkan

Asian Journal of Plant Sciences, 2007, 6(1), 36-41.

Abstract

The experiment was conducted to identify the in vitro responsiveness for callus induction along with plant regeneration of deepwater rice in the presence of NaCl. In this study, callus induction and plant regeneration responsiveness were tested using different concentrations of 0.1-0.3% (w/v) NaCl which added in MS and LS based media. Marked variation was observed both in calli proliferation and plant regeneration among the six deepwater rice cultivars. In the presence of 0.1% (w/v) NaCl, the highest number of callus induction in all genotypes recorded on the media supplemented with 2 mg L^-1 2,4-D + 0.1% (w/v) NaCl. The highest dose of NaCl salt 0.3% (w/v) inhibited callus induction compared to 0.1 and 0.2% NaCl media combinations. Among the cultivars tested, cv. Murabajal produced the highest percentage (39%) of callus whilst cvs. Gheoch (23%) and BR224-2B-2-5 (8%) responded poorly in terms of callus production on the MS based 0.1% NaCl supplemented medium. NaCl had no clear promotive effect on regeneration percentage. In this study, green plant did not regenerate from cv. HA-8. Salinity strongly reduced regenerating capacities of callus obtained from all the cultivars. The mean number of shoots produced per regenerating calli was gradually decreased in all the cultivars when the concentration of NaCl increased in the medium. The apparent tolerance of NaCl in these deepwater rice cultivars may be at least partially related to its growth rate since it can dilute the contents of ion in the shoot. The results of the present study showed the decreasing trend in callus proliferation and plant regeneration with the increasing concentrations of NaCl.

ASCI-ID: 7-1059

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