Research Article 
Identification of RAPD Markers for Northern Corn Leaf Blight Resistance in Waxy Corn (Zea mays var. ceratina)
Juthaporn Khampila, Piyada Theerakulpisut, Kamol Lertrat, Weerasak Saksirirat, Jirawat Sanitchon and Nooduan Muangsan
Asian Journal of Plant Sciences, 2008, 7(1), 18-21.
Abstract
A F2 population from a cross of the waxy corn inbreds 209W and 241 W was used to determine random amplified polymorphic DNA (RAPD) markers linked to Northern Corn Leaf Blight (NCLB) resistance via Bulked Segregant Analysis (BSA). Resistant and susceptible DNA bulks were constructed using the segregating F2 plants based on phenotypic reaction against NCLB infection. In total, two hundred and twenty two decamer primers were used to identify three polymorphic bands observed between the bulks. The primers OPE02, OPJ18 and OPX04, generated the polymorphic DNA fragments of approximately 1200, 650 and 500 bp, respectively. These RAPD bands were present in only in NCLB-resistant bulk and 241W resistant parent. The study revealed that RAPD markers associated to NCLB resistance is potentially useful for the identification of genotyped individuals carrying NCLB resistant traits in breeding programs.
ASCI-ID: 7-1275
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