Research Article 
In vitro Plant Regeneration of an Endangered Sikkim Himalayan Rhododendron (R. maddeni Hook. f.) from Alginate-Encapsulated Shoot Tips
Biotechnology, 2008, 7(1), 144-148.
Abstract
Shoot tips obtained from in vitro cultures of Rhododendron maddeni Hook. f. were precultured on Anderson medium, then encapsulated in sodium alginate hydrogel containing Anderson Medium (AM). Conversion of encapsulated shoot tips into plantlets was significantly affected by the concentration of sodium alginate, duration of storage and planting media with different gelling agent. Amongst the two gelling agents used higher shoot proliferation as well as better growth were observed in cultures grown on phytagel in comparison to agar gelled medium. Encapsulated shoot tips exhibited the best shoot development on AM supplement with 7 mg L-l 2iP, 100 mg L-l PVP, 100 mg L-l ascorbic acid, 10 mg L-l citric acid, 3% sucrose and gelled with 0.3% phytagel. Size of the shoot tips was an important factor in producing multiple shoot. Encapsulated shoot tips demonstrated successful regeneration after different periods of cold storage at 5°C. Among the four different storage conditions (0, 5, 17 and 25°C), the beads stored for 30 days at 5°C showed maximum frequency (68%) of shoot proliferation when placed back to regeneration medium. Shoots regenerated from encapsulated shoot tips required 0.2 mg L-l IBA for rooting. Plantlets developed from encapsulated shoot tips with well developed roots and shoots were transferred successfully to green house. In vivo transplants were observed growing best on peat moss; after one month these were transferred to polythene bags containing normal garden soil, 86% survival was recorded.
ASCI-ID: 11-368
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